The ELISA test was applied to mixed milk from 325 cowsherds of the Landes region of France. From the 88 cowsheds giving a positive or inconclusive response, individual samples of milk were studied by the ELISA test, and individual samples of serum by the agar immunodiffusion test (1734 cows). The same procedure was carried out on 1250 animals in a sample of 49 cowsheds chosen at random from amongst the 237 cowsheds whose mixed milk gave a negative response to the ELISA test. The results confirmed the importance of the ELISA test applied to mixed milks, provided that the samples are studied several times per year, so as to minimize default errors. The study of individual milk allowed the identification of 92 to 94% of the infected animals, with a specificity of 98 to 99%. The systematic and repeated testing of mixed milks by the ELISA test should allow initial detection of infection and supervision of a satisfactory cost-efficiency report in the decontaminated cowsheds.
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