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Leveraging Saccharomyces cerevisiae for ADAR research: From high-yield purification to high-throughput screening and therapeutic applications.
Methods Enzymol
January 2025
Life Science, Bar Ilan University, Ramat Gan, Israel. Electronic address:
Saccharomyces cerevisiae, a model eukaryotic organism with a rich history in research and industry, has become a pivotal tool for studying Adenosine Deaminase Acting on RNA (ADAR) enzymes despite lacking these enzymes endogenously. This chapter reviews the diverse methodologies harnessed using yeast to elucidate ADAR structure and function, emphasizing its role in advancing our understanding of RNA editing. Initially, Saccharomyces cerevisiae was instrumental in the high-yield purification of ADARs, addressing challenges associated with enzyme stability and activity in other systems.
View Article and Find Full Text PDFA photodetector for red and green with balanced negative and positive photocurrent for imaging is realized.
Sci Rep
January 2025
Guangdong Provincial Key Laboratory of Optical Information Materials and Technology, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou, 510006, People's Republic of China.
A dual-polarity, photovoltaic photodetector for red-green dual-wavelength detection is demonstrated, operating in the self-powered mode. It is based on a core-shell n-InGaN nanowire/p-CuO heterostructure with inner upward energy band bending and near surface downward energy band bending. This produces negative photocurrent for red light illumination and positive photocurrent for green light illumination.
View Article and Find Full Text PDFHigh-performance triboelectric nanogenerators based on Ag-doped ZnO loaded electrospun PVDF nanofiber mats for energy harvesting and healthcare monitoring.
Sci Rep
January 2025
Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, India.
This study investigates the potential of zinc oxide (ZnO) and Ag-doped zinc oxide (Ag-ZnO) nanoparticles (NPs) (1, 3 and 5 wt%) electrospun into poly(vinylidene fluoride) (PVDF) based triboelectric nanogenerators (TENGs) to harness electrical energy from ambient mechanical vibrations. ZnO and Ag-ZnO NPs were developed using a co-precipitation method. 3 wt% Ag-ZnO doping was optimized to exhibit a higher β-crystalline phase in PVDF (PAZ3).
View Article and Find Full Text PDFScrutinizing the untapped potential of emerging ABSe (A = Ca, Ba; B = Zr, Hf) chalcogenide perovskites solar cells.
Sci Rep
January 2025
Facultad de Química, Materiales-Energía, Universidad Autónoma de Querétaro, Santiago de Querétaro, C.P.76010, Querétaro, México.
ABSchalcogenide perovskites (CPs) are emerging as promising alternatives to lead halide perovskites due to their unique properties. However, their bandgap exceeds the Shockley-Queisser limit. By substituting S with Se, the bandgap is significantly reduced, shifting it from the visible into the near-infrared region.
View Article and Find Full Text PDFCattle on the rocks: Understanding cattle mobility, diet, and seasonality in the Iberian Peninsula. The Middle Neolithic site of Cova de les Pixarelles (Tavertet, Osona).
PLoS One
January 2025
School of History Archaeology and Religion, Cardiff University, Cardiff, United Kingdom.
Reconstructing past herd mobility, reproduction, and diet is crucial for understanding animal management practices among the first sedentary farming communities. It can also shed light on how domestic animals were integrated into the existing exchange networks of goods, products, and raw materials, and how they contributed to broader economic and social changes during the Neolithic. Despite the longstanding importance of cattle (Bos taurus) to herders, the role of cattle in the daily, seasonal, and annual cycle of activities of early farming communities remains relatively poorly understood.
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