Elevated Microsatellite Alterations at Selected Tetranucleotide repeats (EMAST) occur in up to 60% of colorectal cancers and may associate with aggressive and advanced disease in patients. Although EMAST occurs in many cancer types, current understanding is limited due to the lack of an animal model. Reported here is the design and implementation of an algorithm for detecting EMAST repeats in mice. This algorithm incorporates properties of known human EMAST sequences to identify repeat sequences in animal genomes and was able to identify EMAST-like sequences in the mouse. Seven of the identified repeats were analyzed further in a colon cancer mouse model and six of the seven displayed EMAST instability characteristic of that seen in human colorectal cancers. In conclusion, the algorithm developed successfully identified EMAST repeats in an animal genome and, for the first time, EMAST has been shown to occur in a mouse model of colon cancer.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137214 | PMC |
| http://dx.doi.org/10.1038/s41598-018-32057-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Tumor Mutation Signature Reveals the Risk Factors of Lung Adenocarcinoma with or Mutation.
Cancer Control
January 2025
School of Basic Medical Sciences, Anhui Medical University, Hefei, China.
Introduction: and mutations are frequently detected in lung adenocarcinoma (LUAD). Tumor mutational signature (TMS) determination is an approach to identify somatic mutational patterns associated with pathogenic factors. In this study, through the analysis of TMS, the underlying pathogenic factors of LUAD with and mutations were traced.
View Article and Find Full Text PDFElevated Microsatellite Alterations at Selected Tetranucleotide Repeats (EMAST) in Penile Squamous Cell Carcinoma-No Evidence for a Role in Carcinogenesis.
Curr Oncol
September 2024
Institute of Pathology, University Hospital Erlangen-Nürnberg, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany.
Penile squamous cell carcinoma (pSCC) is a rare malignancy with a global incidence ranging from 0.1 to 0.7 per 100,000 males.
View Article and Find Full Text PDFUnique miRNA Expression Profile in MSI- and EMAST-Unstable Sporadic Colon Cancer.
Genes (Basel)
August 2024
Laboratory for Personalized Medicine, Division of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, Croatia.
MicroRNAs (miRNAs) are critical post-transcriptional gene regulators and their involvement in sporadic colon cancer (CRC) tumorigenesis has been confirmed. In this study we investigated differences in miRNA expression in microsatellite stable (MSS/EMAST-S), microsatellite unstable marked by high elevated microsatellite alterations at selected tetranucleotide repeats (MSS/EMAST-H), and high microsatellite unstable (MSI-H/EMAST-H) tumor subgroups as well as in tumors with different clinicopathologic characteristics. An RT-qPCR analysis of miRNA expression was carried out on 45 colon cancer and adjacent normal tissue samples (15 of each group).
View Article and Find Full Text PDFMolecular dynamics and experimental analysis of energy behavior during stress relaxation in magnetorheological elastomers.
Sci Rep
August 2024
Department of Production Engineering, Faculty of Technology, Tomas Bata University in Zlín, 760 01, Zlín, Czech Republic.
Article Synopsis
- The study investigates the performance and failure resistance of magnetorheological elastomers (MRE), focusing on how stress relaxation affects their molecular and mechanical properties.
- The research utilizes molecular dynamics simulations to better understand the stress relaxation process in MREs made with varying amounts of magnetic iron, finding a significant decrease in stored energy across all models.
- Results indicate that the MRE with 80 wt% magnetic iron exhibits the highest final stored energy due to stronger molecular interactions, highlighting the potential for MD simulations as a valuable tool in MRE material design and failure prevention.
Differential expression of angiogenesis-related genes 'VEGF' and 'angiopoietin-1' in metastatic and EMAST-positive colorectal cancer patients.
Sci Rep
May 2024
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abnormal angiogenesis leads to tumor progression and metastasis in colorectal cancer (CRC). This study aimed to elucidate the association between angiogenesis-related genes, including VEGF-A, ANGPT-1, and ANGPT-2 with both metastatic and microsatellite alterations at selected tetranucleotide repeats (EMAST) subtypes of CRC. We conducted a thorough assessment of the ANGPT-1, ANGPT-2, and VEGF-A gene expression utilizing publicly available RNA sequencing and microarray datasets.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!