AI Article Synopsis
- The study focuses on the process of decidualization, which is crucial for embryo implantation and the formation of the placenta, by analyzing the transcriptome and epigenome profiles of endometrial stromal cells (ESCs).
- ESCs were collected from human tissue and subjected to a decidualization process using specific treatments for 4 and 8 days, revealing significant changes in epigenetic patterns, particularly with H3K27ac, which correlated with gene expression.
- The research identified specific genes that were upregulated or downregulated during decidualization, providing insight into the molecular mechanisms at play, and creating a valuable dataset for further studies on this process.
Article Abstract
Aim: Decidualization is essential for embryo implantation and placental development. We aimed to obtain transcriptome and epigenome profiles for primary endometrial stromal cells (ESCs) and in vitro decidualized cells.
Materials & Methods: ESCs isolated from human endometrial tissues remained untreated (D0), or decidualized for 4 days (D4) and 8 days (D8) in the presence of 8-bromo-cAMP and progesterone.
Results: Among the epigenetic modifications examined (DNA methylation, H3K27ac, H3K9me3 and H3K27me3), the H3K27ac patterns changed most dramatically, with a moderate correlation with gene expression changes, upon decidualization. Subsets of up- and down-regulated genes upon decidualization were associated with reciprocal changes of H3K27ac and H3K27me3 modifications at their promoter region, and were enriched with genes essential for decidualization such as WNT4, ZBTB16, PROK1 and GREB1.
Conclusion: Our dataset is useful to further elucidate the molecular mechanisms underlying decidualization.
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| http://dx.doi.org/10.2217/epi-2018-0006 | DOI Listing |
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