The present study was conducted to systematically investigate the optimal viral titer as well as the volume of the adenovirus vector (ADV) that expresses -actinin-AcGFP in the Z-disks of myocytes in the left ventricle (LV) of mice. An injection of 10 L ADV at viral titers of 2 to 4 × 10 viral particles per mL (VP/mL) into the LV epicardial surface consistently expressed -actinin-AcGFP in myocytes , with the fraction of AcGFP-expressing myocytes at ~10%. Our analysis revealed that SL was ~1.90-2.15 m upon heart arrest via deep anesthesia. Likewise, we developed a novel fluorescence labeling method of the T-tubular system by treating the LV surface with CellMask Orange (CellMask). We found that the T-tubular distance was ~2.10-2.25 m, similar to SL, in the healthy heart . Therefore, the present high-precision visualization method for the Z-disks or the T-tubules is beneficial to unveiling the mechanisms of myocyte contraction in health and disease .
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| http://dx.doi.org/10.1155/2018/4349170 | DOI Listing |
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