AI Article Synopsis
- The study aimed to develop a real-time quantitative PCR method to detect the β-actin gene in Psammosilene tunicoides, which can be used as a reference for analyzing other genes through qPCR.
- Specific primers were designed to amplify the β-actin gene, resulting in a 153 bp amplicon that showed high genetic similarity to β-actin from other plant species.
- The results indicated that β-actin is a stable reference gene across different tissues of Psammosilene tunicoides, making it suitable for researching genes related to triterpenoid saponin biosynthesis.
Article Abstract
Objective: To establish a real-time quantitative PCR method to detect Psammosilene tunicoides β-actin, and to provide a reference gene for the detection of Psammosilene tunicoides genes by q PCR.
Methods: Specific primers were designed based on the conserved region of the β-actin gene( Gen Bank) and were used to amplify β-actin by PCR. β-actin was also used as a reference gene in the q PCR analysis of glycosyltransferase gene( UGT) expression in the roots,stems,and leaves of Psammosilene tunicoides.
Results: The length of the β-actin gene amplicon from Psammosilene tunicoides was 153 bp and shared relatively high homology with β-actin found in Vaccaria segetalis, Myosoton aquaticum and Portulaca oleracea. Furthermore, UGT was revealed to be stably expressed in different Psammosilene tunicoides tissues when β-actin was employed as the reference gene.
Conclusion: β-actin is a reliable and suitable reference gene for studies on the expression of triterpenoid saponin biosynthesis-related genes in Psammosilene tunicoides.
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