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Store-Operated Ca Entry in Drosophila Primary Neuronal Cultures. | LitMetric

AI Article Synopsis

  • Intracellular calcium signals in neurons are triggered by G protein-coupled receptors (GPCRs) when neurotransmitters and neuropeptides bind to them, leading to the production of inositol 1,4,5-trisphosphate (IP).
  • This IP activates specific receptors in the endoplasmic reticulum (ER) that release calcium (Ca) from ER stores, and in Drosophila, depleting these stores can cause store-operated Ca entry (SOCE) through proteins STIM and Orai.
  • The study of this Ca signaling pathway in Drosophila is facilitated by genetic manipulation, allowing researchers to investigate protein functions, and it includes methods for measuring calcium release and SO

Article Abstract

Intracellular calcium signals in neurons frequently derive from the stimulation of G protein-coupled receptors (GPCR) by neurotransmitters, neuropeptides, and neurohormones. GPCR stimulation in neurons leads to generation of inositol 1,4,5-trisphosphate (IP), which in turn activates endoplasmic reticulum (ER)-localized IP receptors. The IP receptor (IPR) is a ligand-gated Ca channel, which releases Ca from ER stores. In Drosophila neurons it has been shown that depletion of ER Ca store is followed by store-operated Ca entry (SOCE) through STIM and Orai, the ER Ca sensor and the plasma membrane Ca channel respectively. The elucidation of this Ca signaling pathway in neurons has in part been possible due to the ease of genetic manipulation in Drosophila, which has allowed neuron-specific knockdown of various proteins of interest. This has been followed by standardization of conditions for culturing neurons from dissected brains of the relevant genotypes, such that they could be used for robust Ca measurements by imaging with standard Ca indicator dyes. Protocols for measurement of IP-mediated Ca release, passive depletion of ER Ca store, and SOCE in primary cultures of Drosophila neurons are described here.

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Source
http://dx.doi.org/10.1007/978-1-4939-8704-7_11DOI Listing

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