Store-operated Ca entry (SOCE) is a Ca influx pathway at the plasma membrane that replenishes intracellular Ca stores in response to depletion of Ca stores. The SOC current, also known as the Ca release-activated Ca current (I), has a small conductance, which makes selective recording difficult. This challenge may be addressed using techniques based on identification of Ca influx patch-clamp electrophysiological recording and measurement of cytoplasmic Ca accumulation with Ca-sensitive fluorophores. Here, we describe specific methods for studying SOCE using these approaches in rat dorsal root ganglion neurons.
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