The aim of this paper is to study the molecular mechanism of Shaofu Zhuyu decoction in treating dysmenorrhea of endometriosis based on GPER2/MAPK/STAT1 axis. In this study, HE staining was used to observe the pathological changes of the rats in each group. The levels of TNF-α and IL-6 were detected by ELISA assay. The mRNA expressions of neurotransmitter receptor (NK1) and GPER were detected by qPCR. The protein contents of MAPK and STAT1 were detected by Western blot. According to the results, compared with the model group, Shaofu Zhuyu decoction could significantly improve the inflammation of the ectopic uterine cavity tissue, decrease the contents of TNF-α and IL-6 in the uterine cavity, the mRNA expressions of NK1 and GPER, and the protein expressions of MAPK and STAT1. In conclusion, Shaofu Zhuyu decoction could effectively inhibit the expressions of GPER2, MAPK and STAT1, decrease the levels of TNF-α, IL-6 and NK1 mRNA and relieve the inflammatory pain in patients with endometriosis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.19540/j.cnki.cjcmm.20180408.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of Shaofu Zhuyu decoction on intestinal flora and fibrosis in a mouse model of endometriosis.
Heliyon
October 2024
Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, 100053, China.
Article Synopsis
- Shaofu Zhuyu decoction is commonly used for gynecological conditions, but its effectiveness in treating endometriosis and the mechanisms behind it needed further investigation.
- Researchers analyzed its chemical makeup and tested its effects on endometriosis in mice, observing a significant reduction in the weight of ectopic lesions and improved gut microbiome diversity.
- The treatment also led to lower levels of fibrosis in lesions and changes in specific proteins and metabolic pathways, suggesting that it may alleviate endometriosis through regulating intestinal flora and reducing tissue scarring.
[Mechanism of Liangfang Wenjing Decoction in treatment of hypoxia on endometriosis with cold coagulation and blood stasis by regulating CHCHD4 expression].
Zhongguo Zhong Yao Za Zhi
July 2024
Hebei University of Chinese Medicine Shijiazhuang 050091, China Hebei Traditional Chinese Medicine Formula Granule Technology Innovation Center Shijiazhuang 050091, China Quality Evaluation & Standardization Hebei Province Engineering Research Center of Traditional Chinese Medicine Shijiazhuang 050091, China.
To explore the mechanism of Liangfang Wenjing Decoction regulating coiled-coil-helix coiled-coil-helix domain containing 4(CHCHD4) in the treatment of hypoxia on endometriosis(EMs) with cold coagulation and blood stasis. The rat model of cold coagulation and blood stasis syndrome was prepared by the ice-water bath method, and then the EMs model was established by autologous intimal transplantation. The rats were randomly divided into model group, low, medium, and high(4.
View Article and Find Full Text PDFCombining transcriptomic analysis and network pharmacology to explore the mechanism by which Shaofu Zhuyu decoction improves diabetes mellitus erectile dysfunction.
Phytomedicine
October 2023
Changchun University of Chinese Medicine, Changchun 130117, China; Department of Urology, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China. Electronic address:
Background: Erectile dysfunction is common among the complications of diabetes mellitus. Shaofu Zhuyu decoction (SFZYD) is commonly used to treat diabetic mellitus erectile dysfunction (DMED). However, its main active components and specific mechanism are still unknown.
View Article and Find Full Text PDFThe present study aimed to investigate the protective role of Shaofu Zhuyu Decoction(SFZY) against endometriosis fibrosis in mice, and decipher the underlying mechanism through the phosphatase and tensin homolog deleted on chromosome ten(PTEN)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR) pathway. Eighty-five BALB/c female mice were randomly assigned into a blank group, a model group, high-, medium, and low-dose SFZY(SFZY-H, SFZY-M, and SFZY-L, respectively) groups, and a gestrinone suspension(YT) group. The model of endometriosis was induced by intraperitoneal injection of uterine fragments.
View Article and Find Full Text PDF[Mechanism of Shaofu Zhuyu Decoction in treatment of endometriosis-associated dysmenorrhea with syndrome of cold coagulation and blood stasis based on MSK1/2].
Zhongguo Zhong Yao Za Zhi
September 2022
Clinical College of Chinese Medicine, Gansu University of Chinese Medicine Lanzhou 730000, China.
This study aims to decipher the mechanism underlying the effect of Shaofu Zhuyu Decoction on endometriosis(EMT)-associated dysmenorrhea in rats with the syndrome of cold coagulation and blood stasis based on mitogen-and stress-activated protein kinase 1/2(MSK1/2).We employed a random number table to randomly assign SPF female non-pregnant rats into the sham group, and treated the rest rats with autologous transplantation+refrigerator freezing for the modeling of the syndrome of cold coagulation and blood stasis.The modeled rats were then randomly assigned into the control group and high-, medium-and low-dose Shaofu Zhuyu Decoction groups.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!