Molecular and Physio-Biochemical Characterization of Cotton Species for Assessing Drought Stress Tolerance.

Drought stress significantly limits cotton growth and production due to the necessity of water at every stage of crop growth. Hence, it is essential to identify tolerant genetic resources and understand the mechanisms of drought tolerance in economically and socially important plants such as cotton. In this study, molecular and physio-biochemical investigations were conducted by analyzing different parameters by following standard protocols in three different cotton species, namely TM-1 (), Zhongmian-16 (), and Pima4-S (). Drought stress significantly decreased plant growth, chlorophyll content, net photosynthetic rate (), stomatal conductance (), maximum photochemical efficiency of PSII (/), and relative water content. TM-1 resulted in more tolerance than the other two species. The accumulation of proline, soluble proteins, soluble sugars, hydrogen peroxide (H₂O₂), and superoxide radicals (O₂) increased significantly in TM-1. In addition, TM-1 maintained the integrity of the chloroplast structure under drought conditions. The relative expression level of drought-responsive genes including coding for transcription factors and other regulatory proteins or enzymes controlling genes (, , , , , , , , , and ) were higher in TM-1 under drought, conferring a more tolerant status than in Zhongmian-16 and Pima4-S. The findings of this research could be utilized for predicting a tolerant cotton genotype as well as evaluating prospective cotton species in the variety development program.