The hemolytic and sphingomyelinase C activities of supernatants of cultures of Leptospira interrogans serovar pomona tended to copurify when isoelectric fractionation was carried out. Both activities focused primarily at pH 8.1. Considered in conjunction with other circumstantial evidence, the results led to the conclusion that sphingomyelinase C is responsible for hemolysis.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC260148PMC
http://dx.doi.org/10.1128/iai.54.1.262-264.1986DOI Listing

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