The hemolytic and sphingomyelinase C activities of supernatants of cultures of Leptospira interrogans serovar pomona tended to copurify when isoelectric fractionation was carried out. Both activities focused primarily at pH 8.1. Considered in conjunction with other circumstantial evidence, the results led to the conclusion that sphingomyelinase C is responsible for hemolysis.
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http://dx.doi.org/10.1128/iai.54.1.262-264.1986 | DOI Listing |
Microorganisms
December 2024
Department of Entomology, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand.
Leptospirosis, a global zoonotic disease caused by spp., presents high morbidity and mortality risks, especially in tropical regions like Thailand. Military personnel deployed in endemic areas, such as during the Cobra Gold Joint exercise, face heightened exposure.
View Article and Find Full Text PDFHeliyon
January 2025
Bacterial Disease Laboratory, Postgraduate Program in Animal Science in Tropics - Federal University of Bahia, Salvador, Bahia, Brazil.
Leptospirosis is a zoonotic infectious disease that significantly impacts animal and public health. Comparative genomics can aid in understanding poorly understood aspects of leptospirosis pathogenesis, including infection mechanisms, antimicrobial resistance, and host interactions across different epidemiological scenarios. This study aimed to compare the genomes of serogroup Icterohaemorrhagiae strains isolated from three host species in a single epidemiological scenario.
View Article and Find Full Text PDFVet World
November 2024
School of Veterinary Medicine, National University of Loja (UNL), Loja 1101608, Ecuador.
Background And Aim: Leptospirosis is an infectious zoonotic disease that significantly affects animal health, particularly the reproduction of ruminants. However, some aspects of epidemiology and clinical characteristics have not been clarified. This study aimed to estimate the prevalence and identify risk factors of leptospirosis in female bovines at reproductive age in the Ecuadorian Amazon rainforest.
View Article and Find Full Text PDFEcohealth
January 2025
Department of Agricultural and Environmental Sciences, State University of Santa Cruz, Direction of the University: Campus Soane Nazaré de Andrade, Rod. Jorge Amado, Km 16 - Salobrinho, Ilhéus, Bahia, 45662-900, Brazil.
The black rat Rattus rattus is an exotic and synanthropic rodent prominent in Brazil and with high adaptation to urban areas. The species have an omnivorous diet feed on human food resources, potentially becoming infected and spreading infectious agents that cause zoonoses such as leptospirosis, leishmaniosis, Chagas disease, and toxoplasmosis, which are significant public health concerns in the country. We analyzed the epidemiologic profile of R.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Molecular Crop, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address:
A dihydrofolate reductase (DHFR)-like enzyme from Leptospira interrogans (LiDHFRL) was cloned and the recombinant protein was characterized. Sequence alignment suggested that the enzyme lacked the conserved catalytic residues found in DHFR. Indeed, LiDHFRL did not catalyze the reduction of dihydrofolate by either NADH or NADPH.
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