AI Article Synopsis
Article Abstract
FTO, the first RNA demethylase discovered, mediates the demethylation of internal N-methyladenosine (mA) and N, 2-O-dimethyladenosine (mA) at the +1 position from the 5' cap in mRNA. Here we demonstrate that the cellular distribution of FTO is distinct among different cell lines, affecting the access of FTO to different RNA substrates. We find that FTO binds multiple RNA species, including mRNA, snRNA, and tRNA, and can demethylate internal mA and cap mA in mRNA, internal mA in U6 RNA, internal and cap mA in snRNAs, and N-methyladenosine (mA) in tRNA. FTO-mediated demethylation has a greater effect on the transcript levels of mRNAs possessing internal mA than the ones with cap mA in the tested cells. We also show that FTO can directly repress translation by catalyzing mA tRNA demethylation. Collectively, FTO-mediated RNA demethylation occurs to mA and mA in mRNA and snRNA as well as mA in tRNA.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151148 | PMC |
| http://dx.doi.org/10.1016/j.molcel.2018.08.011 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cellular functions and biomedical applications of circular RNAs.
Acta Biochim Biophys Sin (Shanghai)
December 2024
Shool of Life Science, Southern University of Science and Technology, Shenzhen 518055, China.
Circular RNAs (circRNAs) have emerged as a large class of stable and conserved RNAs that are derived primarily from back-splicing of pre-mRNAs and expressed in a cell- and tissue-specific fashion. Recent studies have indicated that a subset of circRNAs may undergo translation through cap-independent pathways mediated by internal ribosome entry sites (IRESs), m6A modifications, or IRES-like short elements. Considering the stability and low immunogenicity of circRNAs, transcribed circRNAs hold great promise in biomedical applications.
View Article and Find Full Text PDFStrongyloidiasis mimics duodenal lymphoma in a patient with systemic lupus erythematosus and antiphospholipid syndrome: a case report.
J Med Case Rep
December 2024
Department of Gastroenterology, Shahid Mohammadi Hospital, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Background: Systemic lupus erythematosus is a multi-organ autoimmune disorder that is treated by immunosuppressive agents that weaken the immune defense against opportunistic pathogens and latent infections such as strongyloidiasis. Herein, we report the case of a 43-year-old woman known to have systemic lupus erythematosus who presented with gastrointestinal symptoms, edema, and bone pain 2 months after receiving immunosuppressive treatment.
Case Presentation: A 43-year-old Iranian female known to have systemic lupus erythematosus and antiphospholipid syndrome presented with abdominal pain, nausea, vomiting, and generalized edema.
Accuracy of Controlled Attenuation Parameter for Liver Steatosis in High-Risk Patients for MASLD Using MRI-Proton Density Fat Fraction as Reference Standard.
Dig Dis Sci
December 2024
Second Medical Department, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Aim: Controlled attenuation parameter (CAP) enables the noninvasive diagnosis of liver steatosis. Magnetic resonance imaging proton density fat fraction (MRI-PDFF) is increasingly used over biopsy for the assessment of steatosis in patients at risk for metabolic dysfunction-associated steatotic liver disease (MASLD). We assessed the accuracy of CAP for liver steatosis defined as MRI-PDFF ≥ 5%.
View Article and Find Full Text PDFRelationship Between Coronary Artery Calcium Score and Vulnerability of Culprit Plaque Assessed by OCT in Patients With Established Coronary Artery Disease.
Circ Cardiovasc Imaging
December 2024
Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston. (D.F., D.K., K.S., T.N., M.C., I.M., I.-K.J.).
Background: Coronary artery calcium score (CACS) is widely used for risk stratification. However, in patients with established coronary artery disease, its clinical implication and relationship with plaque vulnerability are unclear. We sought to correlate the CACS and plaque vulnerability assessed by optical coherence tomography.
View Article and Find Full Text PDFA Machine Learning-Based Prediction Model for Acute Kidney Injury in Patients With Community-Acquired Pneumonia: Multicenter Validation Study.
J Med Internet Res
December 2024
Department of Nephrology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
Background: Acute kidney injury (AKI) is common in patients with community-acquired pneumonia (CAP) and is associated with increased morbidity and mortality.
Objective: This study aimed to establish and validate predictive models for AKI in hospitalized patients with CAP based on machine learning algorithms.
Methods: We trained and externally validated 5 machine learning algorithms, including logistic regression, support vector machine, random forest, extreme gradient boosting, and deep forest (DF).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!