COMPARISON OF THE SHAPE OF RABBIT MESENCHYMAL STROMAL CELLS DURING FIVE PASSAGES AFTER PRODUCTION OF PRIMARY CULTURE.

Cultivation of cells under artificial conditions is one of the necessary procedures when working with stem cells. At this stage, the cells lose control by the macroorganism, becoming independent systems. Therefore, during the passaging of the cells, the probability of undesirable modifications is increased. At present, there is not enough data on the first indications about modification of the cultured cells and when they can appear. In the work, the rabbit medullary mesenchymal stromal cells (MSCs) were studied during 5 passages after producing primary culture. The area and the spreading coefficient of a cell were used to evaluate the cell state. The measurements were made 1 h and 1 day after cell reseeding by analyzing digital images of intact living cells. During this time interval, the absolute values of the cell area increased with the passage number, whereas the increment of the cell growth area did not depend on the passage number. It is possible to distinguish three groups of cells. Smaller cells (the 1st group) prevail in cell population of the 1st passage. Next, their proportion is decreased and the proportion of larger cells is increased. Passaging of the cells does not influence their spreading coefficient.