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Comet-like Heterodimers "Gold Nanoflower @Graphene Quantum Dots" Probe with FRET "Off" to DNA Circuit Signal "On" for Sensing and Imaging MicroRNA In Vitro and In Vivo. | LitMetric

Comet-like Heterodimers "Gold Nanoflower @Graphene Quantum Dots" Probe with FRET "Off" to DNA Circuit Signal "On" for Sensing and Imaging MicroRNA In Vitro and In Vivo.

Anal Chem

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control , Jiangnan University, Wuxi , Jiangsu 214122 , P. R. China.

Published: October 2018

AI Article Synopsis

Article Abstract

Cardiovascular diseases have recently become the number one cause of death worldwide and the risk of getting cardiovascular diseases is doubled as the age increases. MicroRNA-34a (miRNA-34a) as an important potential sensor of aging and cellular senescence could be used in early diagnostics. Herein, a new ultrasensitive platform on the basis of the fluorescence resonance energy transfer (FRET) "off" to DNA circuit signal "on" principle was established, termed comet-like heterodimers gold nanoflower (AuNF) @ graphene quantum dots (GQDs) probe. We discussed that the distance of 4 nm between AuNF and GQDs would increase fluorescence quenching efficiency, and light up sensitivity after the probe combined with a target miRNA initiating DNA circuit strategy. The target miRNA-34a can be quantified down to 0.1 fM, which is about 2 orders of magnitude lower than the existing sensing protocols. Furthermore, we constructed the aging myocardial cell and animal model, and the nanoprobe presented low cytotoxicity and satisfied signal imaging in vitro and in vivo. Significantly, this platform herein is envisioned to provide a reliable guidance for early diagnosing cardiovascular diseases and proposing therapeutic protocols.

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Source
http://dx.doi.org/10.1021/acs.analchem.8b02854DOI Listing

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