AI Article Synopsis
- The oligonucleotide d(CTCTCT) exhibits a parallel double helix structure at low pH according to 500 MHz 1H NMR studies.
- This unique configuration includes cytosine-cytosine base pairs and thymine-thymine bulges.
- These characteristics may contribute to the increased sensitivity of S1 nuclease to supercoiled plasmids that contain d(CT)n inserts when in acidic conditions.
Article Abstract
500 MHz 1H NMR studies using 2D-NOESY indicate that the oligonucleotide d(CTCTCT) at low pH forms a parallel double helix with cytosine . cytosine base pairs and thymine . thymine bulges. This unusual structure may explain the hypersensitivity of S1 nuclease at low pH towards supercoiled plasmids containing d(CT)n inserts.
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| http://dx.doi.org/10.1016/0014-5793(86)80902-4 | DOI Listing |
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