AI Article Synopsis
- MS-HRM (Methylation Sensitive High-Resolution Melting) is a technique used for assessing DNA methylation, which requires specific primer design recommendations for effective results.
- There was previously no automated software to help design these primers, but the introduction of Softepigen changes that by allowing users to focus on complex genomic regions for better specificity.
- Softepigen has been validated through in-silico PCR analysis, demonstrating satisfactory specificity, and is now available for free use on their website, www.soft-epigen.com.
Article Abstract
Polymerase Chain Reaction (PCR) based techniques for DNA methylation techniques includes the MS-HRM technique. Methylation Sensitive High-Resolution Melting (MS-HRM) primer-design requires a set of necessary recommendations for such DNA methylation assessment. However, there were not any available software that allows an automatic design of this kind primers. We present Softepigen, the first complete MS-HRM primer design software. Softepigen allows to search for primers in a genomic region following Wojdacz's recommendations and targets primer binding regions with high linguistic complexity sequences that increase the specificity of the converted sequence of the human genome. We performed in-silico PCR analysis through BiSearch ePCR tool to validate the specificity of the of the primers designed using Softepigen. Softepigen for MS-HRM performance in our genomic regions of interest show satisfactory specificity measurements, and we implemented it for freely available use in the web-based interface at www.soft-epigen.com.
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| http://dx.doi.org/10.1109/TCBB.2018.2867600 | DOI Listing |
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