AI Article Synopsis
- Researchers have made significant progress in techniques for manipulating brain circuitry but struggle to analyze peptide release in living organisms.
- A new system has been created that merges in vivo optogenetics with microdialysis.
- This innovative approach employs a sensitive mass spectrometry-based assay to measure the release of opioid peptides in freely moving rodents.
Article Abstract
Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135606 | PMC |
| http://dx.doi.org/10.7554/eLife.36520 | DOI Listing |
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