AI Article Synopsis

  • Ion-beam milling of frozen, hydrated protein crystals creates thin lamella that maintain crystal structure at near-atomic resolution.
  • This technique helps in determining protein structures by connecting the scale between small nanometer crystals and larger micron-sized crystals suitable for advanced imaging.
  • The ability to retain atomic detail implies that this milling method could also yield similar information for samples taken from frozen cells.

Article Abstract

We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffraction and micron scale of synchrotron microfocus beamlines. The demonstration that atomic information can be retained suggests that milling could provide such detail on sections cut from vitrified cells.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156647PMC
http://dx.doi.org/10.1073/pnas.1809978115DOI Listing

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