This work reports on fully integrated "sample-in-signal-out" microfluidic paper-based analytical devices (μPADs) relying on bioluminescence resonance energy transfer (BRET) switches for analyte recognition and colorimetric signal generation. The devices use BRET-based antibody sensing proteins integrated into vertically assembled layers of functionalized paper, and their design enables sample volume-independent and fully reagent-free operation, including on-device blood plasma separation. User operation is limited to the application of a single drop (20-30 μL) of sample (serum, whole blood) and the acquisition of a photograph 20 min after sample introduction, with no requirement for precise pipetting, liquid handling, or analytical equipment except for a camera. Simultaneous detection of three different antibodies (anti-HIV1, anti-HA, and anti-DEN1) in whole blood was achieved. Given its simplicity, this type of device is ideally suited for user-friendly point-of-care testing in low-resource environments.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282528 | PMC |
| http://dx.doi.org/10.1002/anie.201808070 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Resonance SERS probe based on the bifunctional molecule IR808 combined with SA test strips for highly sensitive detection of monkeypox virus.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
Clinical Research Institute, Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, School of Medicine, College of Chemistry and Chemical Engineering, College of Energy, College of Physical Science and Technology, and Discipline of Intelligent Instrument and Equipment, Xiamen University, Xiamen 361005 China; Scientific Research Foundation of State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Xiamen 361005 China. Electronic address:
As a zoonotic virus, highly sensitive detection of monkeypox virus is crucial for its prevention and control due to its rapid increase in cases worldwide and the extremely high risk of virus transmission. In this paper, based on the principle of antigen-antibody specific recognition, an ultrasensitive resonance Raman biosensing probe was prepared using a molecule with the bifunctionality of resonance Raman effect and capturing antibody; and with the strong affinity of the biotin-streptavidin (Bio-SA) system, Bio-antibody and SA test strips were prepared. To match the T-line of the test strip, a portable Raman instrument with a strip-shaped spot was designed.
View Article and Find Full Text PDFSalivary Cortisol Detection with a Fully Inkjet-Printed Paper-Based Electrochemical Sensor.
Micromachines (Basel)
October 2024
Instituto de Microelectrónica de Barcelona IMB-CNM (CSIC), Campus Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain.
Electrochemical paper-based analytical devices (ePADs) offer an innovative, low-cost, and environmentally friendly approach for real-time diagnostics. In this study, we developed a functional all-inkjet paper-based electrochemical immunosensor using gold (Au) printed ink to detect salivary cortisol. Covalent binding of the cortisol monoclonal antibody onto the printed Au surface was achieved through electrodeposition of 4-carboxymethylaniline (CMA), with ethanolamine passivation to prevent non-specific binding.
View Article and Find Full Text PDFElectrochemical and optical biosensors for the detection of E. Coli.
Clin Chim Acta
January 2025
Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
E. coli is a common pathogenic microorganism responsible for numerous food and waterborne illnesses. Traditional detection methods often require long, multi-step processes and specialized equipment.
View Article and Find Full Text PDFComparison of protein immobilization methods with covalent bonding on paper for paper-based enzyme-linked immunosorbent assay.
Anal Bioanal Chem
November 2024
Department of Chemistry, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama, 700-8530, Japan.
In this study, two methods were examined to optimize the immobilization of antibodies on paper when conducting a paper-based enzyme-linked immunosorbent assay (P-ELISA). Human IgG, as a test-capture protein, was immobilized on paper via the formation of Schiff bases. Aldehyde groups were introduced onto the surface of the paper via two methods: NaIO and 3-aminopropyltriethoxysilane (APTS) with glutaraldehyde (APTS-glutaraldehyde).
View Article and Find Full Text PDFUltrasensitive Rapid Antigen Test by Geometric Lateral Flow Assays and Highly Doped Upconversion Nanoparticles.
Anal Chem
October 2024
Institute for Biomedical Materials and Devices (IBMD), Faculty of Science, University of Technology Sydney, Ultimo 2007, New South Wales, Australia.
The paper-based lateral flow assay (LFA) testing strips are currently the most widely used for point-of-care testing (POCT), valued for their rapid result turnaround times in a few minutes. However, their sensitivity has been limited. Upconversion nanoparticles (UCNPs), especially highly doped ones, have emerged as promising luminescent reporters to enhance the LFA sensitivity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!