Comparison of efficacy of local anesthetic solution, distilled water and normal saline as emergency fixatives.

Context: Adequate tissue fixation is fundamental to good quality histological sections. Owing to undesirable effects of 10% buffered formalin, its availability in clinics is questionable. Thus, the present study was conducted with a novel approach to fixation, together with the scope of finding fixative properties of more commonly used reagents available at the clinics.

Aims: The present study was aimed to compare the efficacy of local anesthetic solution, normal saline (NS) and distilled water (DW) with that of 10% neutral-buffered formalin.

Settings And Design: It is a single-blinded study where histological assessment of fixation was done to assert if the tissues procured were sufficient or insufficient for the clinical diagnosis with/without any problems.

Subjects And Methods: Forty soft-tissue specimens obtained from 2 goat tongue were used. Tissues each were directly immersed in local anesthesia, DW, NS solution and formalin for 12 and 24 h each and labeled as Group I and Group II, respectively. The sections were evaluated for staining quality and were subjected to statistical analysis.

Statistical Analysis Used: Kruskal-Wallis test was employed to assess the differences in histological quality scores. Comparison between the tissues of the two groups was estimated with Mann-Whitney U-test. Kappa Statistic was used to measure the interobserver variation.

Results: There was a significant difference ( ≤ 0.05) in the efficacy of all the three emergency fixatives.

Conclusions: On the basis of the results obtained, local anesthetic solution can be used as an emergency fixative.