Objective: To investigate the genetic status of Astragalus membranaceuse resources in Gansu province.
Methods: Using SSR molecular marker technology for collection of Astragalus membranaceuse resources for genetic diversity analysis and clustering analysis.
Results: Nine SSR primers were used on PCR amplification of 57 samples in six main areas of Gansu province,PCR products molecular weighted between 100 ~ 500 bp,the polymorphic loci was 82,the polymorphism rate was 97. 56%,and the average polymorphism information content was 0. 438. At the species level,the number of alleles was 1. 976,the effective number of alleles was 1. 459,Nei’ s genetic diversity was 0. 279,Shannon information index was 0. 431,the group of genetic diversity degree was 0. 248,the genetic differentiation among of population was 0. 117,the gene flow coefficient was 3. 775,and the genetic identity was 0. 896 ~ 0. 977.
Conclusion: Astragalus membranaceuse resources of Gansu are relatively pure,and have abundant genetic diversity. The genetic variation mainly comes from the group of inside,the genes communication between populations is frequent,and the kinship between population is consistent with their geographic distance. In addition,the results of cluster analysis showed that the Core SSR primers can distinguish Astragalus and Hedysarum in the similarity of 0. 46,but Astragalus membranaceus var. mongholicus,Astragalus membranaceus and Astragalus tongolensis can’t be distinguished.
Low-frequency non-syndromic hearing loss (LFNSHL) is a rare auditory disorder affecting frequencies ≤ 2000 Hz. To elucidate its genetic basis, we conducted whole-exome sequencing on nine Chinese families (31 affected individuals) with LFNSHL. Four heterozygous pathogenic variants, including two novel variants, were identified in common LFNSHL-related genes (WFS1, DIAPH1) and less common genes (TNC, EYA4), achieving a 44% genetic diagnosis rate.
Increasingly, emerging research evidence has demonstrated that nonalcoholic fatty liver disease (NAFLD) is a disease closely associated with systemic inflammation. However, the specific upstream inflammatory factors engaged in the pathogenesis of NAFLD remain unclear. Our study aimed to identify the inflammatory regulators causally associated with NAFLD pathogenesis through Mendelian randomisation.
The enzyme N-acetyltransferase 2 (NAT2) plays an important role in metabolism and detoxification of xenobiotics, including carcinogens and medications. We aimed to assess the contribution of the NAT2 polymorphism to susceptibility to inflammatory bowel disease (IBD) in the Polish population. The study involved 101 IBD patients and 100 healthy controls.
Despite the remarkable resistance of the nucleic acid phosphodiester backbone to degradation affording genetic stability, the P-O bond must be broken during DNA repair and RNA metabolism, among many other critical cellular processes. Nucleases are powerful enzymes that can enhance the uncatalyzed rate of phosphodiester bond cleavage by up to ∼10-fold. Despite the most well accepted hydrolysis mechanism involving two metals (M to activate a water nucleophile and M to stabilize the leaving group), experimental evidence suggests that some nucleases can use a single metal to facilitate the chemical step, a controversial concept in the literature.
The enormous diversity of bacteriophages and their bacterial hosts presents a significant challenge to predict which phages infect a focal set of bacteria. Infection is largely determined by complementary-and largely uncharacterized-genetics of adsorption, injection, cell take-over, and lysis. Here we present a machine learning approach to predict phage-bacteria interactions trained on genome sequences of and phenotypic interactions among 51 strains and 45 phage λ strains that coevolved in laboratory conditions for 37 days.
