The goal of this study was to establish a system for co-culturing microalgae and crop plants with biogas digestate. We examined growth performances of . and . co-cultured using a commercial liquid fertilizer designed for soilless culture supplemented with vitamins and ammonium. This solution simulated the filtrate of nitrified biogas digestate derived from the organic fraction of municipal solid waste but was supplemented with insufficient plant nutrients (Mg, Fe and Mn). The specific growth rate of the co-cultured . was 0.761 ± 0.081 d (mean ± SE), which was the same rate that achieved when grown as a sole culture. There were no significant differences between . cultured with until the stationary growth phase of was reached and those cultured alone relative to biomass, RGRs (relative growth rates), or relative to SPAD values of leaves. These results suggest that . and . could be co-cultured with the biogas digestate after being nitrified and filtered. In addition, considering concentrations of plant macronutrients in the residual solution after the co-culturing and . , it could be re-used as the nutrient solution for co-culturing and . .
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