AI Article Synopsis
- The study aimed to assess how human digestion affects the effectiveness of antibiotics (tetracycline, ofloxacin, and penicillin) and the resistance levels of Staphylococcus aureus RN4220 and methicillin-resistant S. aureus (MRSA) to these drugs.
- A model simulating the human gastrointestinal tract was used to observe changes in antibiotic concentrations and bacteria resistance during digestion, revealing that antibiotic levels decreased significantly and resistance increased.
- The findings suggest that digestion alters both the efficacy of antibiotics and the resistance of certain bacteria, highlighting important factors in antibiotic treatment effectiveness within the human body.
Article Abstract
Objectives: This study was performed to determine changes in (i) the antimicrobial activity of antibiotics (tetracycline, ofloxacin and penicillin) and (ii) the resistance of Staphylococcus aureus RN4220 (SA RN4220) and methicillin-resistant S. aureus (MRSA) to these antibiotics during in vitro human digestion.
Methods: A human gastrointestinal digestion model simulating the conditions of the mouth, stomach, small intestine and large intestine (with intestinal microbial application) was used in this study. Antimicrobial susceptibility testing was performed by the disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines.
Results: Concentrations of the three antibiotics decreased during digestion. In particular, the three antibiotics were unstable under conditions of stomach to large intestine digestion, and thus a decrease in antibiotic concentration could cause a reduction in antimicrobial activity during in vitro human digestion. The resistance of SA RN4220 and MRSA to the three antibiotics increased after in vitro human digestion. SA RN4220 and MRSA showed less resistance to ofloxacin compared with tetracycline and penicillin during in vitro human digestion.
Conclusions: These results may help to explain the factors affecting antimicrobial activity and resistance to antibiotics during digestion in the human alimentary canal.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jgar.2018.08.017 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!