AI Article Synopsis
- A field survey revealed that symptomatic spinach leaves contained a virus called chickpea chlorotic dwarf virus (CpCDV), linked to visible leaf issues like curling and darkening.
- Researchers used techniques like rolling circle amplification (RCA) and sequencing to confirm the presence of CpCDV and its association with two types of satellite viruses (CLCuMB and CLCuMA).
- This study is noteworthy as it reports, for the first time, the infection of spinach (a dicot plant) by CpCDV and its satellite viruses in Pakistan, contributing to our understanding of plant virus interactions and diseases.
Article Abstract
Spinach is a common vegetable crop and very little data is available about its virus infection. Symptomatic leaves of spinach were collected during field survey. Circular DNA molecules were amplified from symptomatic samples using rolling circle amplification (RCA). After restriction analysis, presumed bands of virus and satellites were cloned, sequenced and analyzed. Analysis of sequenced RCA product revealed the presence of chickpea chlorotic dwarf virus (CpCDV; Mastrevirus). Further analyses of the cloned virus showed that strain "C" of CpCDV was present in symptomatic samples of spinach collected from field associated with vein darkening, curling and enations on leaves. Amplification of alpha- and betasatellites with universal primers was performed. CpCDV showed association with cotton leaf curl Multan betasatellite (CLCuMB) and cotton leaf curl Multan alphasatellites (CLCuMA). Infectivity analysis of CpCDV and CpCDV/CLCuMB were done in N. benthamiana using particle bombardment method and the results showed that CpCDV was able to transreplicates CLCuMB in this host. To our knowledge, this is the first report of a dicot infecting mastrevirus (CpCDV) along with CLCuMB and CLCuMA associated with leaf curl disease of spinach in Pakistan. The significance of the results is discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.virusres.2018.08.017 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
N Protein of Tomato Spotted Wilt Virus Proven to Be Antagonistic Against Tomato Yellow Leaf Curl Virus in Nicotiana benthamiana.
Mol Plant Pathol
January 2025
Facility Horticultural Laboratory of Universities in Shandong, Weifang University of Science and Technology, Shouguang, Shandong, China.
Two phylogenetically unrelated viruses transmitted by different insect vectors, tomato spotted wilt virus (TSWV) and tomato yellow leaf curl virus (TYLCV), are major threats to tomato and other vegetable production. Although co-infections of TSWV and TYLCV on the same host plant have been reported on numerous occasions, there is still lack of research attempting to elucidate the mechanisms underlying the relationship between two viruses when they coexist in the same tomato or other plants. After assessing the effect of four TSWV-coded proteins on suppressing TYLCV in TSWV N transgenic Nicotiana benthamiana seedlings, the TSWV N protein proved to be effective in reducing TYLCV quantity and viral symptoms.
View Article and Find Full Text PDFA novel geminivirus-derived 3' flanking sequence of terminator mediates the gene expression enhancement.
Plant Biotechnol J
December 2024
Key Laboratory of National Forestry and Grassland Administration on Plant Conservation and Utilization in Southern China & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China.
Exploring the new elements to re-design the expression cassette is crucial in synthetic biology. Viruses are one of the most important sources for exploring gene expression elements. In this study, we found that the DNA sequence of the SBG51 deltasatellite from the Sweet potato leaf curl virus (SPLCV) greatly enhanced the gene expression when flanked downstream of the terminator.
View Article and Find Full Text PDFDisease complex associated with begomoviruses infecting squash and cucumber in Saudi Arabia.
Cell Mol Biol (Noisy-le-grand)
November 2024
Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia.
During the field visits in growing season of 2022 in Dammam Region of Saudi Arabia, begomovirus-like symptoms including leaf curling, leaf cupping, leaf distortion, vein thickening and reduced leaf size were observed in squash and cucumber fields. Twenty-five samples were collected from each crop and PCR amplification was done using general diagnostic begomovirus primers (AC-1048/AV-494 and Begomo I/Begomo II). The obtained results showed desired sized amplified DNA fragments (550 bp and 1.
View Article and Find Full Text PDFGenetic diversity and infectivity analysis of tomato yellow leaf curl virus Oman and its associated betasatellite.
Cell Mol Biol (Noisy-le-grand)
November 2024
Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khoud 123, Muscat, Oman.
Tomato yellow leaf curl virus-Oman (TYLCV-OM), a variant of the Tomato yellow leaf curl virus-Iran (TYLCV-IR) strain, was identified in 2005 as the cause of tomato yellow leaf curl disease (TYLCD) in Oman and is associated with a betasatellite namely as Tomato leaf curl betasatellite (ToLCB). Surveys were carried out from three diverse Governorates of Oman to investigate the correlation between the betasatellite and the virus. The visual assessment and scoring of infected tomato plants in the field revealed that the association of betasatellite with the disease was highest in Sharqia at 77%, followed by Dakhlia at41% and lowest in Batinah at30% .
View Article and Find Full Text PDFDeciphering gene specific molecular characterization and partial gene sequence for combined resistance to tomato leaf curl virus (ToLCV) (Ty-1, Ty-2 and Ty-3), fusarium wilt (I-2) and root- knot nematode (Mi-1) in selected fresh market breeding line of tomato (Solanum lycopersicom L.).
Mol Biol Rep
December 2024
Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, (CPMB&B), Tamil Nadu Agricultural University, Coimbatore, 641 037, India.
Background: Tomato (Solanum lycopersicum L.) is a widely cultivated crop in tropical regions, but its production is often hampered by significant losses attributed to diseases like tomato leaf curl virus (ToLCV), fusarium wilt and root-knot nematode.
Methods And Results: This study employed an integrated approach utilizing both co-dominant and dominant SCAR markers, selected for specific resistance genes (ToLCV-Ty-1, Ty-2, Ty-2, Fusarium wilt (Race-2)-I-2, and Root-knot nematode-Mi-1.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!