Assessing reproducibility and the effects of demographic variables on the normal macular layers using the Spectralis SD-OCT.

Purpose: To quantify and view the possible influence of demographic variables on normal macular layers. Additionally, we wanted to assess the reproducibility using the Spectralis SD-OCT.

Methods: A Spectralis SD-OCT machine using a commercially available algorithm was used to scan 242 healthy subjects in an outpatient setting. We examined retinal thicknesses in seven layers: retinal nerve fiber layer (RNFL), ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL) and retinal pigment epithelium (RPE). Combined retinal thicknesses were expressed as inner retinal layer (IRL), photoreceptor layer (PL) and total retinal thickness (TRT). Measurements were taken from each of the nine sectors defined by the Early Treatment Diabetic Retinopathy Study; the center was the fovea, the inner circle (IC) was 1-3 mm away, and the outer circle (OC) was 3-6 mm away.

Results: The TRT was thickest inferiorly in the IC, and superiorly in the OC. The RNFL (=0.030), GCL (=0.006), IPL (=0.006), IRL (=0.030), PL (<0.001) and TRT (=0.001) were found to be thicker in males. The GCL (=0.078, =0.001), IPL (=0.079, =0.001), IRL (=0.072, =0.002), PL (=0.076, =0.001) and TRT (=0.090, <0.001) were found to decrease with age. The INL (=0.060, =0.010), ONL (=0.078, =0.001), and RPE (=0.066, =0.004) were inversely related to axial length. Excellent reproducibility was observed in all layers.

Conclusion: Our study shows differences in various retinal layers according to age, gender, and axial length. Additionally, we demonstrate excellent reproducibility of this algorithm using the Spectralis SD-OCT.