AI Article Synopsis
- A study on invasive pulmonary aspergillosis (IPA) highlights the role of microRNAs (miRNAs) in its regulation, particularly in immunocompromised patients with high mortality rates.
- Researchers created a mouse model for IPA and used next-generation sequencing to analyze small RNA transcriptomes in lung tissues, discovering a total of 3759 known miRNAs, with 23 specifically linked to IPA infection.
- Eight of these miRNAs were validated, showing consistent expression changes that may regulate important components of the NF-kappa B signaling pathway, enhancing understanding of IPA pathogenesis and the complexity of small RNAs in immune responses.
Article Abstract
Background: Invasive pulmonary aspergillosis (IPA) is a severe opportunistic infection with high mortality in patients with compromised immunity. The full repertoire of microRNAs (miRNAs) involved in the regulation of IPA infection remains to be established.
Methods: We established a mouse IPA model and analyzed small RNA transcriptomes in lung tissues of immunodeficient IPA mice (IPA group) and matched immunodeficient control mice (control group) through next-generation sequencing.
Results: A total of 3759 known miRNAs were detected, in which 23 miRNAs were identified to be related to IPA. IPA-associated miRNAs include upregulated mmu-let-7b-3p, mmu-miR124-3p, mmu-miR21a-3p, mmu-miR29c-5p, mmu-miR3473b and mmu-miR3473e, and downregulated mmu-miR-150-3p and mmu-miR-503-5p. The expression levels of eight identified miRNAs were quantified in a validation cohort (n = 40) by qRT-PCR, and results revealed the same change patterns. MiRNA target prediction revealed that all IPA-related miRNAs possibly engage a cooperative regulation of key elements in the NF-kappa B signaling pathway.
Conclusion: We conclude that deep-sequencing small RNAs can uncover miRNA pool-regulating IPA. Our results may lead to further understanding IPA pathogenesis and gain insight into the complexity and diversity of small RNA molecules that regulate immunodeficient IPA.
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| http://dx.doi.org/10.1016/j.jmii.2016.09.002 | DOI Listing |
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