It was done the polyphasic taxonomic analysis of carotene synthesizing strain Bacillus sp. 1.1, perspective for the creation of probiotic. The investigated strain was referred to the species Bacillus subtilis based on of cultural-morphological, physiological and biochemical features. Despite this the fatty acid profile of the cell walls of this strain is predominantly presented by branched derivatives of iso- and antiiso C15:0 and C17:0 compounds (over 70 %), which is typical for the species Bacillus amyloliquefaciens. Based on the nucleotide sequence of 16S rRNA gene, and the profile of polymorphic nucleotides strain was referred to the species Bacillus amyloliquefaciens.
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