AI Article Synopsis
- Polyethylenimine (PEI) is a cationic polymer important for developing non-viral gene delivery systems, with significant potential in gene therapies.
- The study focused on examining how branched PEI (0.8 kDa) distributes in zebrafish embryos after being exposed to it for 24 and 48 hours.
- Results indicated that PEI effectively penetrated various layers of the embryos, showing significant distribution in the yolk and body parts after prolonged exposure, especially in the first 24 hours.
Article Abstract
Background: Polyethylenimine (PEI) plays important roles in the pharmaceutical design of non-viral gene delivery systems. Due to a set of unique physicochemical properties this cationic polymer has a great potential in modern gene therapies.
Objective: The aim of the present study was to determine the distribution of branched PEI (0.8 kDa) in zebrafish embryos (Danio rerio).
Material And Methods: Zebrafish embryos at 3 hours post-fertilization (hpf) were incubated with PEI (10 μg/ml) for 24 and 48 hours and studied using the confocal laser microscopy.
Results: The obtained results show that PEI effectively distributed into the layers of the chorion and yolk sac in developing embryos due to first 24 hours of exposure. In contrast, PEI was found in the yolk, head, trunk and tail of the embryos due to prolonged treatments (48 hours).
Conclusion: The study evidences a high distribution of the branched PEI (0.8 kDa) polymer in the zebrafish embryo tissues.
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