AI Article Synopsis
- The study examined how embryonic thermal manipulation affects mRNA levels and antioxidant capacity in broiler chickens exposed to heat stress.
- Cobb and Hubbard breeds of chickens were divided into control and thermal manipulation groups, with the latter incubated at higher temperatures and humidity for part of their development.
- Results indicated that TM chickens showed lower expression of specific genes related to oxidative stress and a reduced antioxidant capacity under acute heat stress, suggesting TM may enhance long-term heat tolerance in these breeds.
Article Abstract
Effects of embryonic thermal manipulation (TM) on mRNA expressional levels and total antioxidant capacity of genes associated with heat-induced oxidative stress (NOX4, GpX2, SOD2, catalase, and AvUCP) in 2 breeds of broiler chicken were investigated. Fertile Cobb and Hubbard eggs (n = 1,200) were divided into 4 treatment groups: Cobb control, Cobb TM, Hubbard control, and Hubbard TM. Control groups were maintained under standard conditions (37.8°C; 56% relative humidity), whereas TM groups were incubated at 39°C and 65% relative humidity for 18 h a day from embryonic days (ED) 10 to 18. On post-hatch day 28, the broilers were subject to acute heat stress (AHS) at 40°C for 7 h. At certain intervals (0, 1, 3, 5, and 7 h), 12 chickens from each of the 4 groups were humanely euthanized, and liver samples were immediately isolated. During AHS, in both breeds, the mRNA expression levels of NOX4, GPx2, SOD2, and catalase in TM chickens were significantly lower than in controls, but AvUCP mRNA expression in the TM group was higher. The total antioxidant capacity and activity of superoxidase dismutase and catalase were significantly lower in the TM than in the control group in both breeds. The results of this study suggest that TM has a long-lasting effect on the acquisition of thermotolerance in 2 broiler chicken breeds as indicated by the reduction of system genes associated with heat-induced oxidative stress.
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| http://dx.doi.org/10.3382/ps/pey379 | DOI Listing |
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