[Preparation of zirconia nanoparticles deposited cotton fibers and their application in the rapid enrichment of phosphopeptides].

A kind of cotton-based fibrous adsorbent, zirconia nanoparticles coated cotton fiber (ZrO-CF), was successfully prepared by liquid-phase deposition of zirconia nanoparticles on the surface of cotton fiber. And the ZrO-CF fibrous adsorbent was packed in pipet tips for the enrichment of phosphopeptide by pipetting up and down within 2 min, which was simple and fast. Nine phosphopeptides were identified from the digestion of -casein after ZrO-CF extraction. ZrO-CF exhibited good selectivity in the enrichment of phosphopeptides from the digestion mixture of -casein and BSA (molar ratio is 1:100) in which four phosphopeptides were identified. And it also showed excellent extraction efficiency in actual biological samples such as non-fat milk and human serum in which four and nine phosphopeptides were identified, respectively.