Introduction: Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times.
Methods: This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction.
Results: The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations.
Conclusions: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols.
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| http://dx.doi.org/10.1590/0037-8682-0352-2017 | DOI Listing |
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