The pattern of glucose repression in most Kluyveromyces marxianus strains does not correlate with fermentative behaviour; however, glucose repression and fermentative metabolism appear to be linked to the kinetics of sugar uptake. In this work, we show that lactose transport in K. marxianus CCT 7735 by lactose-grown cells is mediated by a low-affinity H-sugar symporter. This system is glucose repressed and able to transport galactose with low affinity. We also observed the activity of a distinct lactose transporter in response to raffinose. Regarding glucose uptake, specificities of at least three low-affinity systems rely on the carbon source available in a given growth medium. Interestingly, it was observed only one high-affinity system is able to transport both glucose and galactose. We also showed that K. marxianus CCT 7735 regulates the expression of sugar transport systems in response to glucose availability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10482-018-1143-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Porungo cheese whey: a new substrate to produce β-galactosidase.
An Acad Bras Cienc
November 2023
Federal University of Rio Grande do Sul, Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Food Science and Technology Institute, Av. Bento Gonçalves, 9500, 91501-970 Porto Alegre, RS, Brazil.
The bioconversion of porungo cheese whey to produce β-galactosidase in batch system was studied. The whey released after curd cutting and precipitation during porungo cheese production was collected in borosilicate flasks. Two strains of Kluyveromyces marxianus, CCT 4086 and CBS 6556, and whey supplementation with different nitrogen sources were evaluated.
View Article and Find Full Text PDFScreening and Growth Characterization of Non-conventional Yeasts in a Hemicellulosic Hydrolysate.
Front Bioeng Biotechnol
April 2021
Institute of Technology, University of Tartu, Tartu, Estonia.
Lignocellulosic biomass is an attractive raw material for the sustainable production of chemicals and materials using microbial cell factories. Most of the existing bioprocesses focus on second-generation ethanol production using genetically modified , however, this microorganism is naturally unable to consume xylose. Moreover, extensive metabolic engineering has to be carried out to achieve high production levels of industrially relevant building blocks.
View Article and Find Full Text PDFAdaptive responses of Kluyveromyces marxianus CCT 7735 to 2-phenylethanol stress: Alterations in membrane fatty-acid composition, ergosterol content, exopolysaccharide production and reduction in reactive oxygen species.
Fungal Genet Biol
June 2021
Laboratory of Microbial Physiology, Department of Microbiology, Universidade Federal de Viçosa, Viçosa, MG, Brazil. Electronic address:
2-phenylethanol (2-PE) is a higher aromatic alcohol with a rose-like aroma used in the cosmetic and food industries as a flavoring and displays potential for application as an antifungal. Biotechnological production of 2-PE from yeast is an interesting alternative due to the non-use of toxic compounds and the generation of few by-products. Kluyveromyces marxianus CCT 7735 is a thermotolerant strain capable of producing high 2-PE titers from L-Phenylalanine; however, like other yeast species, its growth has been strongly inhibited by this alcohol.
View Article and Find Full Text PDFCorrection to: Transcriptome analysis of the thermotolerant yeast Kluyveromyces marxianus CCT 7735 under ethanol stress.
Appl Microbiol Biotechnol
March 2021
Laboratory of Microbial Physiology, Department of Microbiology, Universidade Federal de Viçosa, Vicosa, MG, Brazil.
Obtainment, selection and characterization of a mutant strain of Kluyveromyces marxianus that displays improved production of 2-phenylethanol and enhanced DAHP synthase activity.
J Appl Microbiol
March 2021
Department of Microbiology, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Aims: Yeasts produce 2-phenylethanol (2-PE) from sugars via de novo synthesis; however, its synthesis is limited due to feedback inhibition on the isofunctional 3-deoxy-d-arabino-heptulosonate-7-phosphate (DAHP) synthases (Aro3p and Aro4p). This work aimed to select Kluyveromyces marxianus mutant strains with improved capacity to produce 2-PE from sugars.
Methods And Results: Kluyveromyces marxianus CCT 7735 mutant strains were selected from UV irradiation coupled with screening of p-fluoro-dl-phenylalanine (PFP) tolerant strains on culture medium without l-Phe addition.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!