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Isolation and characterization of microsatellite loci from (Fabaceae). | LitMetric
Premise Of The Study : Microsatellite primers were developed for a perennial legume from northern China, (Fabaceae), to investigate population genetic structure of this taxon, as well as potential hybridization events with closely related taxa in this genus.Methods And Results : One hundred and five primer pairs were designed from Illumina sequence data and screened for suitability. Fifteen of these primer pairs were polymorphic, and these primers amplified tri-, tetra-, and pentanucleotide repeats with 10-56 alleles per locus. Cross-amplification tests in three other species from northern China (, , and ) revealed that all of these loci can be amplified successfully and show polymorphism.Conclusions : These primer pairs can be used to assess the genetic diversity and population structure in future studies of , as well as studies of potential hybridization events with closely related taxa in this genus.
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