Premise Of The Study: The tetraploid (Plantaginaceae) is a narrow endemic to the Iberian Peninsula. Specific microsatellite markers were developed to investigate genetic structure and diversity.
Methods And Results: A total of 15 polymorphic markers were characterized on three populations of , using a microsatellite-enriched library on an Ion Torrent sequencer and high-resolution melting (HRM) analyses to rapidly discard nonreliable, multicopy, and/or monomorphic loci. Allele number per locus ranged from one to five, and levels of observed heterozygosity per population varied from 0.142 ± 0.301 to 0.281 ± 0.369. Most primers also amplified in the closely related species and in three subspecies of .
Conclusions: The species-specific microsatellite markers developed here represent an essential tool to provide genetic information on the population level for . The low levels of variation detected highlight the importance of continued efforts to improve conservation of the species.
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