Premise Of The Study: Few genetic markers provide phylogenetic information in closely related species of (Isoëtaceae). We describe the development of primers for several putative low-copy nuclear markers to resolve the phylogeny of , particularly in the southeastern United States.
Methods And Results: We identified regions of interest in transcriptomes based on low-copy genes in other plants. Primers were designed for these regions and tested with 16 taxa of and one species of . Parts of the , , and gene regions show phylogenetic signal within the North American and Mediterranean clades of .
Conclusions: Transcriptome data prove useful for identification and primer design of low-copy genes. Three new markers show potential for inferring phylogenies in regional clades of , and possibly across the entire genus.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947607 | PMC |
| http://dx.doi.org/10.1002/aps3.1142 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dryopteris×subdiffracta (Dryopteridaceae), a new natural hybrid fern from Guangxi, China.
PhytoKeys
December 2024
Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China Kunming Institute of Botany, Chinese Academy of Sciences Kunming China.
A new natural hybrid fern, Dryopteris×subdiffracta (Dryopteridaceae), is reported from Guangxi, China. Molecular phylogenetic analysis based on DNA sequences from the low-copy nuclear marker and plastid genome revealed respectively that and are parents of the new hybrid, with as the maternal parent. Cytometric analysis of the nuclear DNA content indicated that might be a diploid hybrid.
View Article and Find Full Text PDFPhylogenomic analyses unravel the tangled evolutionary history of Genisteae (Fabaceae).
Mol Phylogenet Evol
November 2024
Department of Integrative Biology, University of Texas at Austin, Austin, TX, USA.
Article Synopsis
- Genisteae is a tribe in the legume family Fabaceae known for producing quinolizidine alkaloids that help resist pests, but its generic relationships remain unclear due to a lack of modern studies.
- Previous research identified three main clades within Genisteae: Lupinus, Cytisus-Genista, and Argyrolobium, with ongoing debates about the classification of certain genera like Teline and Chamaecytisus.
- Recent phylogenomic analysis, using extensive data from 24 of the tribe's 25 genera, revealed four significant clades within Genisteae, clarified the placement of Teline and Sellocharis, and supported the transfer of the Argyrolobium group into Gen
ExoSloNano: Multi-Modal Nanogold Tags for identification of Macromolecules in Live Cells & Cryo-Electron Tomograms.
bioRxiv
October 2024
School of Biological Sciences, University of California San Diego, La Jolla, CA, USA.
Article Synopsis
- In situ cryo-Electron Microscopy (cryo-EM) allows researchers to study protein structures in their natural cellular environment, significantly advancing the understanding of macromolecular interactions.
- Despite recent advancements, many proteins remain difficult to detect in cryo-EM due to their small size and low abundance, necessitating new methods for observation.
- The introduction of novel nanogold probes for identifying specific proteins in live cells enhances cryo-ET and correlated light and electron microscopy (CLEM), facilitating efficient protein labeling and expanding the range of detectable proteins in cryo-EM studies.
Premise: Target sequence capture (Hyb-Seq) is a cost-effective sequencing strategy that employs RNA probes to enrich for specific genomic sequences. By targeting conserved low-copy orthologs, Hyb-Seq enables efficient phylogenomic investigations. Here, we present Asparagaceae1726-a Hyb-Seq probe set targeting 1726 low-copy nuclear genes for phylogenomics in the angiosperm family Asparagaceae-which will aid the often-challenging delineation and resolution of evolutionary relationships within Asparagaceae.
View Article and Find Full Text PDFHuman hypofunctional variants promote pulmonary fibrosis in the bleomycin-induced mouse model and patients with systemic sclerosis via expansion of SPP1 monocytes-derived macrophages.
Ann Rheum Dis
September 2024
Division of Rheumatology and Immunology, Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA
Article Synopsis
- The study investigates a genetic variant (p.Arg90His) in the neutrophil cytosolic factor 1 gene related to systemic lupus erythematosus and its association with systemic sclerosis (SSc).
- Data from diverse cohorts indicate that the -H90 allele significantly increases the risk of developing diffuse cutaneous SSc and lung fibrosis in affected patients.
- Findings reveal that this variant enhances profibrotic responses in blood monocytes and macrophages, contributing to the severity of lung fibrosis, both in mouse models and human patients with SSc.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!