Diacylglycerol acyltransferases (DGATs) play a critical role in the biosynthesis of endogenous triglycerides (TGs) and formation of lipid droplets (LDs) in the liver. In particular, one member of DGATs, DGAT-1 was reported to be an essential host factor for the efficient production of hepatitis C virus (HCV) particles. By utilizing our previously characterized three different groups of twelve DGAT inhibitors, we found that one of the DGAT inhibitors, a 2-((4-adamantylphenoxy) methyl)--(furan-2-ylmethyl)-1-benzo[d]imidazole-5-carboxam () is a potent suppressor of both HCV genome replication and particle production. was able to induce inhibition of these two critical viral functions in a mutually separate manner. Abrogation of the viral genome replication by led to a significant reduction in the viral protein expression as well. Interestingly, we found that its antiviral effect did not depend on the reduction of TG biosynthesis by . This suggests that the inhibitory activity of against DGATs may not be directly related with its antiviral action.
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| http://dx.doi.org/10.3390/molecules23082083 | DOI Listing |
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