AI Article Synopsis
- A hybrid mass spectrometry method (LTQ-Orbitrap-HRMS) was employed to analyze myriocin and related compounds in various samples.
- The samples underwent ultrasonic-assisted extraction and purification through ODS solid-phase extraction before being identified using mass spectrometry.
- Findings indicated that myriocin levels were undetectable in wild and artificial samples, but high concentrations were found in certain strains, challenging previous classifications of species in databases like Genbank or Mycobank.
Article Abstract
A hybrid linear ion trap-quadrupole-Orbitrap high-resolution mass spectrometry (LTQ-Orbitrap-HRMS) was used to qualitatively and quantitatively analyse the myriocin in and its allies. The samples were prepared with 95% methanol for 30 min by ultrasonic-assisted extraction. The target compound was purified by ODS solid-phase extraction (SPE) column. The enriched samples were identified by mass spectrometry. The results showed that the contents of myriocin in both wild and artificial were below the detection limit, while a strain of and Shing (Dujiaolong), both closely related to the , and its asexual mycelia are rich in myriocin. It suggests that it may be wrong to consider as 's teleomorph in Genbank or Mycobank in many published reports based on chemical classification, and the species rich in myriocin is probably not .
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059042 | PMC |
| http://dx.doi.org/10.1080/21501203.2017.1383319 | DOI Listing |
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Article Synopsis
- A hybrid mass spectrometry method (LTQ-Orbitrap-HRMS) was employed to analyze myriocin and related compounds in various samples.
- The samples underwent ultrasonic-assisted extraction and purification through ODS solid-phase extraction before being identified using mass spectrometry.
- Findings indicated that myriocin levels were undetectable in wild and artificial samples, but high concentrations were found in certain strains, challenging previous classifications of species in databases like Genbank or Mycobank.
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