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[Toxoplasma gondii Infection in HIV-infected Individuals in Henan Province]. | LitMetric

Genomic DNA was extracted from 1 038 peripheral blood samples from HIV-infected individuals in Henan Province. One-step single-tube nested PCR was performed to amplify the 529 bp repeating sequences of Toxoplasma gondii. Of the 1 038 samples (762 from males and 276 from females), 66 showed positive PCR results, with a positive rate of 6.4%. The PCR positive rate in males and females was 6.3% (48/762) and 6.5% (18/276) respectively. The PCR positive rate in the married HIV individuals was 4.9%(25/508), and that in unmarried, divorced and widowed HIV individuals was 7.7% (41/530)(χ2 = 3.451, P> 0.05). The PCR positive rate in HIV individuals with a high-school educational level or above was 6.9%(34/489), and that in those below the high-school level was 5.8% (32/549)(χ2 = 0.545, P> 0.05). The highest infection rate was in the age group of 20-40 years(7.6%, 31/410). In addition, the Toxoplasma infection rate in those with and without a history of venereal diseases, and those with an unknown history was 8.0%(9/113), 6.5%(50/773) and 4.6%(7/152) respectively (χ2 = 0.355, P> 0.05).

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