Leishmania amazonensis and Leishmania braziliensis are the main causative agents of American Tegumentary Leishmaniasis (ATL) in Brazil. As intracellular parasites, the infection by Leishmania species is dependent on the host immune response and the immunotherapy could be promissory for the development of new strategies to combat ATL. In this work we investigated the leishmanicidal potential of a galactose-binding lectin from the snake venom of Bothrops leucurus (BLL) during the infection with L. amazonensis and L. braziliensis. BLL inhibited the promastigote growth and viability of both species in a mechanism dependent on galactose and calcium. The treatment with BLL also decreases the survival of intracellular parasites for both species and induced profound ultrastructural changes on amastigotes without apparent damage to the host cells. The analysis of the cytokine profile revealed that BLL induced an increase in the proinflammatory cytokines IL-6 and TNF-α by infected macrophages in both species, but differed in relation to IL-1β and IL-10 response. Future works using in vitro and in vivo models are necessary to support the use of these lectins as biotechnological tool in immunological studies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2018.08.064 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Understanding the effects of Bothrops erythromelas and Bothrops leucurus venoms on human blood coagulation.
Toxicon
January 2025
Laboratório de Herpetologia, Instituto Butantan, São Paulo, Brasil; Programa de Pós-Graduação Interunidades em Biotecnologia, São Paulo, Brasil; Laboratório de Fisiopatologia, Instituto Butantan, São Paulo, Brasil. Electronic address:
Coagulation disorders are a primary symptom of envenomation caused by snakes belonging to the genus Bothrops. In the Northeast region of Brazil, the species Bothrops erythromelas and Bothrops leucurus are the main responsible for snakebite accidents. Due to the specific action of Bothrops venoms on several components of the coagulation cascade, the objective of this work was to characterize the coagulotoxic profile of B.
View Article and Find Full Text PDFAssessing the Interactions between Snake Venom Metalloproteinases and Hydroxamate Inhibitors Using Kinetic and ITC Assays, Molecular Dynamics Simulations and MM/PBSA-Based Scoring Functions.
ACS Omega
December 2024
Avenida Antônio Carlos 6627, Dept. De Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
species are the main cause of snake bites in rural communities of tropical developing countries of Central and South America. Envenomation by snakes is characterized by prominent local inflammation, hemorrhage and necrosis as well as systemic hemostatic disturbances. These pathological effects are mainly caused by the major toxins of the viperidae venoms, the snake venom metalloproteinases (SVMPs).
View Article and Find Full Text PDFAnalysis of the genus snake venom: An inter and intraspecific comparative study.
Heliyon
September 2024
Laboratório de Herpetologia, Instituto Butantan, São Paulo, Brazil.
Article Synopsis
- * Researchers analyzed venom from eight adult snakes using various methods, revealing differences in protein profiles and biochemical properties between species and genders.
- * The findings show that male snakes generally had higher LAAO and PLA activities, while females exhibited higher proteolytic activity, and the butropic antivenom was effective against most venom proteins, albeit with some variability.
Anti-inflammatory and antiophidic effects of extract of Hymenaea eriogyne benth and structure-activity relationship prediction of the major markers in silico.
J Ethnopharmacol
December 2024
Laboratory of Pharmacognosy, Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, Brazil. Electronic address:
Ethnopharmacological Relevance: Hymenaea eriogyne Benth (Fabaceae) is popularly known as "Jatobá". Despite its use in folk medicine to treat inflammatory disorders, there are no descriptions that show its anti-inflammatory potential.
Aim Of The Study: In this sense, this study aimed to evaluate the anti-inflammatory and antivenom action of bark and leaves extract of H.
Effect of combined red and infrared wavelengths on inflammation, hemorrhage, and muscle damage caused by Bothrops leucurus snake venom.
Lasers Med Sci
July 2024
Department of Agricultural and Environmental Sciences, Universidade Estadual de Santa Cruz-UESC, Soane Nazaré de Andrade Campus, Ilhéus, BA, Brazil.
To evaluate the effects of red and infrared wavelengths, separately and combined, on the inflammatory process and collagen deposition in muscle damage caused by B. leucurus venom. 112 mice were inoculated with diluted venom (0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!