We have developed a theoretical model for the regenerative amplification of ultrashort mid-infrared radiation in high-pressure transverse electric discharge CO lasers. This model is based on counter-propagating wave equations that are combined with Boltzmann equation, six-temperature, and circuit-coupled kinetic models. For the first time to our knowledge, the effect of spontaneous emission is taken into account. The model describes noise behavior, contrast ratio, and self-lasing of amplified spontaneous emission. The proposed scheme allows the attainment of a few-picosecond pulse with GW-level peak power and contrast ratio on the order of 10 in the 9 μm range.
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http://dx.doi.org/10.1364/AO.57.005249 | DOI Listing |
Planta
January 2025
Advanced Laboratory for Plant Genetic Engineering, Advanced Technology Development Centre, Indian Institute of Technology, Kharagpur, India.
This study seeks to improve the biomass extractability of Sorghum bicolor by targeting a critical enzyme, 4CL, through metabolic engineering of the lignin biosynthetic pathway at the post-transcriptional level. Sorghum bicolor L., a significant forage crop, offers a potential source of carbohydrate components for biofuel production.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Stem Cell Program, Boston Children's Hospital, Boston, MA, USA.
The CRISPR-activated repair lineage tracing (CARLIN) mouse line uses DNA barcoding to enable high-resolution tracing of cell lineages in vivo (Bowling et al, Cell 181, 1410-1422.e27, 2020). CARLIN mice contain expressed barcodes that allow simultaneous interrogation of lineage and gene expression information from single cells.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Department of Anatomy & Embryology, Leiden University Medical Center, Leiden, The Netherlands.
ScarTrace is a CRISPR/Cas9-based genetic lineage tracing method that allows for uniquely barcoding the DNA of single cells at a target GFP sequence during developing zebrafish embryos. Single cells from barcoded adult zebrafish can be isolated from various tissues (e.g.
View Article and Find Full Text PDFBiosensors (Basel)
December 2024
CUHKSZ-Boyalife Regenerative Medicine Engineering Joint Laboratory, School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China.
The Phi29 DNA polymerase is renowned for its processivity in synthesizing single-stranded DNA amplicons by rolling around a circularized DNA template. However, DNA synthesis rolling circle amplification (RCA) is significantly hindered by the secondary structure in the circular template. To overcome this limitation, an engineered circular template without secondary structure could be utilized to improve the sensitivity of RCA-based assays without increasing its complexity.
View Article and Find Full Text PDFBMC Oral Health
December 2024
School of Stomatology, Capital Medical University, Beijing, 100050, China.
Objective: This prospective clinical study examined the effects of fixed orthodontic appliances on oral hygiene and assessed changes in the oral microflora structure of orthodontic patients using high-throughput sequencing technology.
Methods: A total of 45 patients undergoing initial fixed orthodontic treatment were recruited from three hospitals in Beijing, China. Clinical parameters and oral hygiene habits questionnaire were recorded at pre-treatment (T0) and at a 6-month follow-up (T1).
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