In the confocal mode, Raman microspectroscopy can profile the biochemical content of biological cells at a subcellular level, and any changes to it by exogenous agents, such as therapeutic drugs or toxicants. As an exploration of the potential of the technique as a high-content, label-free analysis technique, this report reviews work to monitor the spectroscopic signatures associated with the uptake and response pathways of commercial chemotherapeutic agents and polymeric nanoparticles by human lung cells. It is demonstrated that the signatures are reproducible and characteristic of the cellular event, and can be used, for example, to identify the mode of action of the agent as well as the subsequent cell death pathway, and even mechanisms of cellular resistance. Data mining approaches are discussed and a spectralomics approach is proposed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1364/AO.57.000E11 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Onboard measurement of polyethylene microplastics on a research vessel using Raman micro-spectroscopy: A preliminary study for testing feasibility.
Mar Pollut Bull
January 2025
Faculty of Marine Resources and Environment, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-Ku, Tokyo 108-8477, Japan. Electronic address:
Microplastic pollution in marine environments poses significant environmental risks due to its widespread presence. Traditional micro-imaging measurement of microplastics often rely on post-cruise laboratory analyses. In this study, we explored the feasibility of onboard microplastic measurement using Raman spectroscopy, with a focus on polyethylene (PE).
View Article and Find Full Text PDFCharacterizing the heterogeneous contamination of commercial paper and board food packaging at different scales.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
January 2025
UMR SayFood 0782, Université Paris-Saclay, INRAE, Palaiseau, AgroParisTech, France.
Assessing the contamination of paper and board (P&B) food packaging materials poses significant challenges due to the sensitivity limits of analytical methods and the low precision of sampling processes. This study aims to enhance the understanding of P&B food packaging contamination by investigating the distribution of contaminants at different scales using a combination of chromatographic and spectroscopic techniques. A total of 36 substances were targeted, including phthalates, photoinitiators, and bisphenol A.
View Article and Find Full Text PDFProbing the Local Field Enhancement Using SERS Detection of DNA Strands with Different Lengths and Grafting Strategies on Graphene Oxide Plasmonic Nanoplatforms.
J Phys Chem Lett
January 2025
Biomolecular Physics Department, Faculty of Physics, Babeş-Bolyai University, 1 M. Kogalniceanu Str., 400084 Cluj-Napoca, Romania.
Surface-enhanced Raman spectroscopy (SERS) has emerged as a powerful tool for analyzing nucleic acids due to its exceptional sensitivity and specificity. This study rigorously investigates not only the impact of polyA strands of different lengths (, 5, 10, 15, and 20 adenine bases) but also their distinct grafting strategy (SH at 5' and NH at 5' end) on the SERS signal of DNA strand using synthesized gold nanoparticles (AuNPs) on graphene oxide sheets (GO-AuNPs). By comparing the thiol vs amine bonding onto the GO-AuNP nanoplatform, we found a strong correlation between the adenine peak intensity at 732 cm and the strand length for both grafting methods (SH at 5' end or NH at 5' end).
View Article and Find Full Text PDFPolyethylene microplastic exposure adversely affects oocyte quality in human and mouse.
Environ Int
January 2025
Center for Reproductive Medicine, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, China. Electronic address:
Microplastics (MPs) are pervasive environmental contaminants, resulting in unavoidable human exposure. This study identified MPs in follicular fluid and investigated the specific MPs and mechanisms that adversely affect oocytes. MPs in the follicular fluid of 44 infertile women undergoing assisted reproductive technology were measured using Raman microspectroscopy.
View Article and Find Full Text PDFObservation and Control of Single-Component Adhesion Interphase of Polyamide 66 through Confocal Raman Microspectroscopy.
ACS Appl Mater Interfaces
December 2024
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Rokko, Nada Kobe 657-8501, Japan.
Manufacturing using adhesion technology has attracted much attention. Examples of adhesion include the lay-up of carbon fiber reinforced thermoplastic prepregs and the lamination of food packaging. In single-component adhesion systems, the analysis of the boundary region poses challenges because of the absence of chemical and physical discrimination at the adhesion interphase.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!