Recombinant protein production plays an essential role in both biological studies and pharmaceutical production. is one of the most favorable hosts for this purpose. Although a number of strategies for optimizing protein production have been developed, the effect of gene overexpression on host cell growth has been much less studied. Here, we performed high-throughput tests on the a complete set of K-12 ORF archive (ASKA) collection to quantify the effects of overexpressing individual genes on its growth. The results indicated that overexpressing membrane-associated proteins or proteins with high abundances of branched-chain amino acids tended to impair cell growth, the latter of which could be remedied by amino acid supplementation. Through this study, we expect to provide an index for a fast pre-study estimate of host cell growth in order to choose proper rescuing approaches when working with different proteins.
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| http://dx.doi.org/10.3390/genes9080414 | DOI Listing |
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