The substances decreasing (insulin subcutaneously 30 U/kg single dose) and increasing (isadrin, theophylline orally 30 mg/kg five times with 12-hour intervals) the intracellular level of cAMP exert varying effects of the activities of mono-oxygenases of the rat liver endoplasmic reticulum. Insulin decreases aniline binding with cytochrome P-450 and the rate of its p-hydroxylation (after 6, 12 hours), the content of cytochrome P-450 and the rate of NADP.H oxidation (after 12 hours), the rate of NAD.H oxidation (after 24 hours). The activity of NADP.H-nitrotetrazolium reductase, content of cytochrome B5, the rate of aniline p-hydroxylation are increased by theophylline, and the rate of NADP.H and NAD.H oxidation and the content of cytochrome P-450 are increased by theophylline and isadrin.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Single-nucleotide polymorphisms in genes associated with the vitamin D pathway related to clinical and therapeutic outcomes of American tegumentary leishmaniasis.
Front Cell Infect Microbiol
January 2025
Laboratório de Imunidade Natural (LIN), Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Background: The vitamin D pathway contributes to the microbicidal activity of macrophages against infection. In addition to induction of this pathway, interferon-gamma (IFNγ), interleukin (IL)-15, and IL32γ are part of a network of pro-inflammatory cytokines. The aim of this study was to evaluate single-nucleotide polymorphisms (SNPs) in the components of the vitamin D pathway and associated cytokine genes that could be related to resistance or susceptibility to American tegumentary leishmaniasis (ATL).
View Article and Find Full Text PDFCoordinated conformational changes in P450 decarboxylases enable hydrocarbons production from renewable feedstocks.
Nat Commun
January 2025
Brazilian Biorenewables National Laboratory, Brazilian Center for Research in Energy and Materials, Campinas, SP, Brazil.
Fatty acid peroxygenases have emerged as promising biocatalysts for hydrocarbon biosynthesis due to their ability to perform C-C scission, producing olefins - key building blocks for sustainable materials and fuels. These enzymes operate through non-canonical and complex mechanisms that yield a bifurcated chemoselectivity between hydroxylation and decarboxylation. In this study, we elucidate structural features in P450 decarboxylases that enable the catalysis of unsaturated substrates, expanding the mechanistic pathways for decarboxylation reaction.
View Article and Find Full Text PDF"Brij 35 inhibited the CYP2E1-mediated metabolism and P-gp mediated transport of Paracetamol in rats and models: Amelioration of Paracetamol Toxicity.
Toxicol Mech Methods
January 2025
Department of Pharmacology, Shri Vishnu College of Pharmacy, Vishnupur, Bhimavaram, Andhra Pradesh, India-534202.
The harmful by-product of paracetamol is known as N-Acetyl-p-benzoquinoneimine, (NAPQI). When paracetamol is given at therapeutic dosages or in excess, it undergoes Phase I metabolism in the liver via Cytochrome P-450 2E1 (CYP2E1), and then it produces NAPQI. Previous studies reported that a non-ionic surfactant known as Brij 35 (Polyoxyethylene lauryl ether) has been shown to be an effective inhibitor of CYP2E1 and P-glycoprotein (P-gp).
View Article and Find Full Text PDFGenetics and Pathophysiology of Classic Congenital Adrenal Hyperplasia Due to 21-Hydroxylase Deficiency.
J Clin Endocrinol Metab
January 2025
Division of Pediatric Endocrinology, Department of Pediatrics, UT Southwestern Medical Center, Dallas, TX 75390, USA.
Congenital adrenal hyperplasia (CAH) is an autosomal recessive disease that manifests clinically in varying forms depending on the degree of enzyme deficiency. CAH is most commonly caused by 21-hydroxylase deficiency (21OHD) due to mutations in the CYP21A2 gene. Whereas there is a spectrum of disease severity, 21OHD is generally categorized into 3 forms.
View Article and Find Full Text PDFHeterologous Biosynthesis of Terpenoids in Saccharomyces cerevisiae.
Biotechnol J
January 2025
Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
Terpenoids are widely distributed in nature and have various applications in healthcare products, pharmaceuticals, and fragrances. Despite the significant potential that terpenoids possess, traditional production methods, such as plant extraction and chemical synthesis, face challenges in meeting current market demand. With the advancement of synthetic biology and metabolic engineering, it becomes feasible to construct efficient microbial cell factories for large-scale production of terpenoids.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!