Herbal medicines are complicated chemical systems containing hundreds of small molecules of various polarities, structural types, and contents. Thus far, the chromatographic separation of herbal extracts is still a big challenge. Two-dimensional liquid chromatography (2DLC) has become an attractive separation tool in the past few years. Particularly, a lot of attention has been paid to on-line 2DLC. In this review, we aim to give an overview on applications of on-line 2DLC in the chemical analysis of herbal medicines since 2010. Firstly, classification and general configurations of on-line 2DLC were briefly introduced. Then, we summarized main applications in herbal medicines of heart-cutting 2DLC (LC-LC), comprehensive 2DLC (LC × LC), and their combinations, with emphasis on LC × LC. Mass spectrometry is the most popular detector coupled with 2DLC, which allows sensitive and accurate structural characterization of herbal compounds. Finally, future developments in on-line 2DLC techniques were also discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jpba.2018.08.014 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Phytochemical analysis of ethanolic leaf extract of Cordia myxa and its anti-Inflammatory and cytotoxic activities.
Wiad Lek
January 2025
DEPARTMENT OF PHARMACOLOGY AND TOXICOLOGY, FACULTY OF PHARMACY, UNIVERSITY OF KUFA, KUFA, IRAQ.
Objective: Aim: Testing Cordia myxa extract on colon cancer cell line and caspase-3 gene and COX-2 protein expression.
Patients And Methods: Materials and Methods: This study used Cordia myxa ethanolic extract at various dosages on SW480 cells. Cell proliferation was measured using MTT, also examined effect of Cordia myxa extract on caspase-3 gene expression using quantitative real-time polymerase chain reaction.
Functional Characterization of an Aldol Condensation Synthase PheG for the Formation of Hispidin from Phellinus Igniarius.
Adv Sci (Weinh)
January 2025
College of Pharmacy, Key Laboratory of Protection, Development and Utilization of Medicinal Resources in Liupanshan Area, Ministry of Education, Ningxia Medical University, Yinchuan, 750004, P.R. China.
Hispidin (1) is a polyphenolic compound with a wide range of pharmacological activities that is distributed in both plants and fungi. In addition to natural extraction, hispidin can be obtained by chemical or enzymatic synthesis. In this study, the identification and characterization of an undescribed enzyme, PheG, from Phellinus igniarius (P.
View Article and Find Full Text PDFOccurrence of AG-5 Causing Root Rot on in Northwestern China.
Plant Dis
January 2025
Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu, China;
Astragalus mongholicus is a perennial Chinese medicinal herb in the family Leguminosae widely cultivated in China. In September 2023, A. mongholicus plants in a field in Weiyuan County, Gansu Province, showed symptoms of circular or irregular brown, sunken and necrotic lesions, multiple lesions coalesced, and brown longitudinal cracks in the roots.
View Article and Find Full Text PDFFirst Report of Causing Black Leaf Spot on in China.
Plant Dis
January 2025
Zhejiang Academy of Agricultural Sciences, Institute of Agro-product Safety and Nutrition, Hangzhou, Zhejiang, China;
Chinese yam ( Turcz.), known for its nutrient-rich underground tubers, is both a food source and a traditional Chinese medicinal plant. It offers significant nutritional and medicinal benefits.
View Article and Find Full Text PDFPowdery Mildew of Caused by in China.
Plant Dis
January 2025
Henan Normal University, College of Life Sciences, Xinxiang, Xinxiang, Henan, China, 453007.
Echinacea purpurea (Eastern Purple Coneflower) is a perennial herbaceous plant belonging to the Asteraceae. It originated from North America and is cultivated all over the world. Extracts of E.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!