(Sw.), DC induces apoptosis and cell cycle arrest in triple negative breast cancer cells: and investigations.

Plant originated drugs/formulations are extensively prescribed by the physicians as a complementary therapy for treating various human ailments including cancer. In this study leaves methanol extract was prepared and exposed to human breast cancer cell lines i.e. MDA-MB-231 and MCF-7 and human keratinocytes HaCaT as a representative of normal cells. Initially, a series of experiments like cell proliferation, migration, colony formation, cell cycle arrest and inhibition of angiogenesis. After confirmation of the efficient and selective activity against triple negative breast cancer cell line, we further evaluated the possible mechanism of inducing cell death and experiments like detection of reactive oxygen species, caspases and poly (ADP-ribose) polymerase cleavage study and Annexin V assay were performed. We also evaluated anti tumorigenic activity of the leaves by using 4T1 cells (a triple negative mouse origin breast cancer cell line) and BALB/c xenograft mouse model. experiments revealed that methanol extract of leaves possess impressive anti-breast cancer activity more specifically against triple negative breast cancer cells, while the studies demonstrated that leaves extract significantly suppressed the 4T1 induced tumor growth. Present investigations clearly focus the significance of as an important resource for finding novel leads against triple negative breast cancer. The results may also act as a ready reference towards developing based formulation as an alternative and complementary medicine for the management of breast cancer.