AI Article Synopsis
- Cryopreservation of platelets (PLTs) is being studied as a strategy to address platelet shortages, using a method that involves storing them at -80°C with dimethyl sulphoxide (DMSO).
- An experiment involved 49 pooled platelet concentrates (BC-PLTs) from 245 healthy donors, evaluating their count, viability, and function at various time intervals over 9 months.
- Results showed a significant decrease in specific platelet markers after storage, but the cryopreserved platelets maintained good viability and thrombin generation potential throughout the study period.
Article Abstract
Cryopreservation for the long-term storage of platelets (PLTs) is a useful method to overcome the limits of platelet shortage. This is an in vitro prospective study to evaluate the count, viability, and function of buffy coat-derived pooled platelet concentrates (BC-PLTs), treated with dimethyl sulphoxide (DMSO) and cryopreserved (CRY BC-PLTs) at -80 °C with a modified Valeri method. PLTs were stored in 6% DMSO with a patented kit. Overall, 49 BC-PLTs from 245 healthy volunteer donors were prepared, cryopreserved, and analysed before and after 3, 6, and 9 months of storage. In flow cytometry, a statistically significant reduction in CD 42b (92.7 ± 4.29% at T0 vs. 23.6 ± 27.5% at T3, 16.38 ± 12.54% at T6, and 17.3 ± 9.6% at T9) and PAC-1 (1.9 ± 1.34% at T0 vs. 0.62 ± 0.4% at T3, 0.63 ± 0.83% at T6, and 0.49 ± 0.48% at T9) was observed after storage. CRY BC-PLTs showed a good and stable endogenous thrombin generation potential (nM min): 529.25 ± 98.64 at T0 vs. 533.04 ± 103.15 at T9 months. CRY BC-PLTs showed a good viability in vitro, according to currently accepted criteria for cryopreserved PLTs.
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| http://dx.doi.org/10.1016/j.transci.2018.07.020 | DOI Listing |
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Article Synopsis
- Cryopreservation of platelets (PLTs) is being studied as a strategy to address platelet shortages, using a method that involves storing them at -80°C with dimethyl sulphoxide (DMSO).
- An experiment involved 49 pooled platelet concentrates (BC-PLTs) from 245 healthy donors, evaluating their count, viability, and function at various time intervals over 9 months.
- Results showed a significant decrease in specific platelet markers after storage, but the cryopreserved platelets maintained good viability and thrombin generation potential throughout the study period.
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