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Energy Resolved Mass Spectrometry Data from Surfaced Induced Dissociation Improves Prediction of Protein Complex Structure.
Anal Chem
January 2025
Department of Chemistry and Biochemistry, Ohio State University, Columbus, Ohio 43210, United States.
Native Mass Spectrometry (nMS) is a versatile technique for elucidating protein structure. Surface-Induced Dissociation (SID) is an activation method in tandem MS predominantly employed for determining protein complex stoichiometry alongside information about interface strengths. SID-nMS data can be collected over a range of acceleration energies, yielding Energy Resolved Mass Spectrometry (ERMS) data.
View Article and Find Full Text PDFDissecting Key Factors Influencing Electrochemical Conversion of Carbon Solutions over Nickel Single Atoms.
Small
January 2025
Environment Research Institute, Shandong University, Qingdao, 266237, China.
The direct electrochemical conversion of bicarbonate solutions (i.e., captured CO) has emerged as a sustainable approach for integrating CO capture and utilization compared to the traditional independent and sequential route.
View Article and Find Full Text PDFPrediction of Cyclic O Molecules Stabilized by Helium under Pressure.
Adv Sci (Weinh)
January 2025
Center for High-Pressure Science, State Key Laboratory of Metastable Materials Science and Technology, School of Science, Yanshan University, Qinhuangdao, 066004, China.
Oxygen usually exists in the form of diatomic molecules at ambient conditions. At high pressure, it undergoes a series of phase transitions from diatomic O to O cluster and ultimately dissociates into a polymeric O spiral chain structure. Intriguingly, the commonly found cyclic hexameric molecules in other group VIA elements (e.
View Article and Find Full Text PDFEnhancing CO Adsorption on MgO: Insights into Dopant Selection and Mechanistic Pathways.
Biomimetics (Basel)
December 2024
Entropic Interface Group, Engineering Product Development, Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore.
Inspired by our recent success in designing CO-phobic and CO-philic domains on nano-MgO for effective CO adsorption, our ongoing efforts focus on incorporating dopants into pristine MgO to further enhance its CO adsorption capabilities. However, a clear set of guidelines for dopant selection and a holistic understanding of the underlying mechanisms is still lacking. In our investigation, we combined first-principles calculations with experimental approaches to explore the crystal and electronic structural changes in MgO doped with high-valence elements (Al, C, Si, and Ti) and their interactions with CO.
View Article and Find Full Text PDFReaction and interaction dynamics of azobenzene-tethered DNA with T7 RNA polymerase.
Phys Chem Chem Phys
January 2025
Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Reaction and interaction dynamics of azobenzene-tethered DNA (photoresponsive DNA) with T7 RNA polymerase (T7RNAP) were studied after photoisomerization of azobenzene from the - to -forms using the transient grating (TG) and time-resolved fluorescence polarization techniques. Two types of photoresponsive DNA were examined: AzoPBD, tethered at the protein binding site, and AzoTATA, tethered at the unwinding site. A diffusion change was observed after photoexcitation of -AzoPBD within 1 ms, and this change is explained in terms of a structural change from a bent to an extended conformation upon the -to- photoisomerization.
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