The DNA from flying squirrel-associated Rickettsia prowazekii was characterized by using a specific DNA fragment produced by digestion with the enzyme BamHI. The DNA fragment was cloned into a plasmid vector and used to readily distinguish between available human- and flying squirrel-associated R. prowazekii DNAs derived from crude cytoplasmic extracts.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC268600 | PMC |
http://dx.doi.org/10.1128/jcm.23.1.189-191.1986 | DOI Listing |
Emerg Infect Dis
October 2003
Centers for Disease Control and Prevention, 1600 Clifton Road, Mail Stop G13, Atlanta, GA 30338, USA.
In March 2002, typhus fever was diagnosed in two patients residing in West Virginia and Georgia. Both patients were hospitalized with severe febrile illnesses, and both had been recently exposed to or had physical contact with flying squirrels or flying squirrel nests. Laboratory results indicated infection.
View Article and Find Full Text PDFThe DNA from flying squirrel-associated Rickettsia prowazekii was characterized by using a specific DNA fragment produced by digestion with the enzyme BamHI. The DNA fragment was cloned into a plasmid vector and used to readily distinguish between available human- and flying squirrel-associated R. prowazekii DNAs derived from crude cytoplasmic extracts.
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