A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085288 | PMC |
| http://dx.doi.org/10.1038/s41598-018-30644-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Predictive value of trendelenburg position and carotid ultrasound for fluid responsiveness in patients on VV-ECMO with acute respiratory distress syndrome in the prone position.
Sci Rep
December 2024
Department of Critical Care Medicine, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou, 324000, Zhejiang, China.
Fluid administration is widely used to treat hypotension in patients undergoing veno-venous extracorporeal membrane oxygenation (VV-ECMO). However, excessive fluid administration may lead to fluid overload can aggravate acute respiratory distress syndrome (ARDS) and increase patient mortality, predicting fluid responsiveness is of great significance for VV-ECMO patients. This prospective single-center study was conducted in a medical intensive care unit (ICU) and finally included 51 VV-ECMO patients with ARDS in the prone position (PP).
View Article and Find Full Text PDFVariation of gene ratios in mock communities constructed with purified 16S rRNA during processing.
Sci Rep
December 2024
Department of Chemical Engineering, Polytechnic School, University of São Paulo, Av. Prof. Luciano Gualberto, Travessa 3, n. 380., São Paulo, SP, CEP 05508-900, Brazil.
16S ribosomal nucleic acid (16S rRNA) analysis allows to specifically target the metabolically active members of microbial communities. The stability of the ratios between target genes in the workflow, which is essential for the bioprocess-relevance of the data derived from this analysis, was investigated using synthetic mock communities constructed by mixing purified 16S rRNA from Bacillus subtilis (Bs), Staphylococcus aureus (Sa), Pseudomonas aeruginosa (Pa), Klebsiella pneumoniae (Kp) and Burkholderia cepacia (Bc) in different proportions. The RT reaction yielded one copy of cDNA per rRNA molecule for Pa, Bc and Sa but only 2/3 of the expected cDNA from 16S rRNAs of Bs and Kp.
View Article and Find Full Text PDFPrediction correction modeling of water-conducting fracture zones height due to repeated mining in close distance coal seams.
Sci Rep
December 2024
School of Minging and Geomatics Engineering, Hebei University of Engineering, Handan, 10076, China.
The height of the water-conducting fracture zones (WCFZ) is crucial for ensuring safe coal mining beneath aquifers, particularly considering the secondary development of the WCFZ in upper seams due to repeated mining in close distance coal seams. Accurately predicting this height is essential for mine safety, groundwater protection, and optimal coal resource use. This study compiles extensive measured data from various mining areas in China to analyze the coupling relationship between the WCFZ development height and six influencing factors: mining thickness, mining depth, coal seam spacing, hard rock lithology ratio, and the slope length of working face.
View Article and Find Full Text PDFOrthogonal and multiplexable genetic perturbations with an engineered prime editor and a diverse RNA array.
Nat Commun
December 2024
Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, USA.
Programmable and modular systems capable of orthogonal genomic and transcriptomic perturbations are crucial for biological research and treating human genetic diseases. Here, we present the minimal versatile genetic perturbation technology (mvGPT), a flexible toolkit designed for simultaneous and orthogonal gene editing, activation, and repression in human cells. The mvGPT combines an engineered compact prime editor (PE), a fusion activator MS2-p65-HSF1 (MPH), and a drive-and-process multiplex array that produces RNAs tailored to different types of genetic perturbation.
View Article and Find Full Text PDFPatient-specific cutting guides allow 1° precision in asymmetric anterior closing-wedge osteotomy.
J Exp Orthop
January 2025
Department of Trauma and Orthopaedics, Institute for Locomotion, Sainte-Marguerite Hospital Aix-Marseille University Marseille France.
Purpose: Asymmetric anterior closing-wedge high tibial osteotomy (ACWHTO) allows correction of both excessive posterior tibial slope (PTS) and varus deformity. However, the complexity of this surgery requires a high degree of accuracy, which is less likely to be achieved with standard instrumentations. This study aimed to determine the accuracy of 3D patient-specific cutting guides (PSCGs) to provide the accurate planned correction in the frontal and sagittal planes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!