Corn DDGS is poorly digested by pigs. Pretreatment or predigestion of whole stillage (WS; slurry material from which DDGS is derived) can potentially improve corn DDGS digestibility. Thus, a study was conducted to determine the effects of pretreating WS with heat (160 °C and 70 psi for 20 min) alone or in combination with citric acid (10 g/L; CA), sulfuric acid (90 mM; HSO), or ammonia (1%), without or with subsequent multienzymatic hydrolysis for 24 h, on porcine digestibility. Dried untreated, heat-pretreated, CA-pretreated, HSO-pretreated, and ammonia-pretreated WS contained 23, 21, 12 19, and 18% total nonstarch polysaccharides, respectively. Pretreatment increased in vitro digestibility of dry matter of WS by ∼11 (CA) to ∼15% units (ammonia). Multienzyme hydrolysis increased in vitro digestibility of dry matter of WS by ∼6 (ammonia-treated WS) to ∼18% units (untreated WS). Thus, pretreatment or predigestion can improve the digestibility of WS and hence the resulting DDGS.
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http://dx.doi.org/10.1021/acs.jafc.8b01943 | DOI Listing |
Environ Res
June 2023
Applied Microbiology Laboratory, Center for Rural Development and Technology, Indian Institute of Technology- Delhi, New Delhi, 110016, India.
A green extraction method is developed using partially hydrolysed microalgal cells grown in biocompatible solvent for simultaneous cultivation and extraction of bioproducts from a highly efficient permeabilized microalgal cell with enhanced biomass and lipid content for potential use in biodiesel production. Incomplete digestion of cell wall was achieved by regulating the incubation time of the enzymatic pretreatment of the microalgal cells. 15.
View Article and Find Full Text PDFFood Chem
April 2023
Quadram Institute Bioscience, Norwich Research Park, Colney, Norwich NR4 7UA, UK. Electronic address:
Wheat is the staple crop for 35% of the world's population, providing a major source of calories, mainly in the form of starch. The digestibility of wheat starch varies between different flours and products. Wheat products that are rapidly digested elicit large post-prandial glucose peaks associated with metabolic disorders.
View Article and Find Full Text PDFGenes (Basel)
April 2022
Centre for Palaeogenetics, Svante Arrhenius väg 20C, 10691 Stockholm, Sweden.
Rapid and cost-effective retrieval of endogenous DNA from ancient specimens remains a limiting factor in palaeogenomic research. Many methods have been developed to increase ancient DNA yield, but modifications to existing protocols are often based on personal experience rather than systematic testing. Here, we present a new silica column-based extraction protocol, where optimizations were tested in controlled experiments.
View Article and Find Full Text PDFJ Chromatogr B Analyt Technol Biomed Life Sci
April 2022
Department of Pharmacy & Pharmacology, Antoni van Leeuwenhoek/The Netherlands Cancer Institute, Amsterdam, The Netherlands; Division of Pharmacoepidemiology and Clinical Pharmacology, Faculty of Science, Department of Pharmaceutical Sciences, Utrecht University, The Netherlands.
Ipilimumab, nivolumab, and pembrolizumab are immune checkpoint inhibiting monoclonal antibodies. Their efficacy has been proven to be correlated with clearance, and hence, bioanalytical assays to study their pharmacokinetics are of pivotal importance. We present the first kit-free sample pre-treatment procedure of only three hours for the Ultra-Performance Liquid Chromatography - tandem Mass Spectrometry (UPLC-MS/MS) simultaneous quantification of ipilimumab, nivolumab, and pembrolizumab in human serum.
View Article and Find Full Text PDFFront Microbiol
August 2020
Faculty of Science and Engineering, Centre for Healthy Eating and Food Innovation, Maastricht University, Maastricht, Netherlands.
The aim of the study was to investigate the effect of untreated and processed rapeseed meal (RSM) on fiber degradability by pig gut microbiota and the adaptation of the microbiota to the substrate, by using the Swine Large Intestine Model (SLIM). A standardized swine gut microbiota was fed for 48 h with pre-digested RSM which was processed enzymatically by a cellulase (CELL), two pectinases (PECT), or chemically by an alkaline (ALK) treatment. Amplicons of the V3-V4 region of the 16S rRNA gene were sequenced to evaluate the gut microbiota composition, whereas short chain fatty acids (SCFA) were measured to assess fiber degradation.
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